Carbon-felt-based Bioelectrocatalytic Flow-detectors: Optimization of the Adsorption Conditions of Horseradish Peroxidase and Thionine onto Carbon-felt for Highly Sensitive Amperometric Determination of H2O2

• WANG Yue

  Department of Materials Science and Engineering, Graduate School of Engineering, Saitama Institute of Technology School of Chemical Engineering, University of Science and Technology Liaoning

• HASEBE Yasushi

  Department of Materials Science and Engineering, Graduate School of Engineering, Saitama Institute of Technology Department of Life Science and Green Chemistry, Saitama Institute of Technology

抄録

Horseradish peroxidase (HRP) and thionine (Th) were co-adsorbed onto a porous carbon-felt (CF) from a mixed solution of HRP and Th to fabricate a CF-based bioelectrocatalytic flow-detector for H₂O₂. The resulting HRP and Th co-adsorbed CF (HRP/Th-CF) was successfully used as a working electrode unit of a highly sensitive amperometric flow-H₂O₂-biosensor. Differing from the ordinary mediator-based HRP biosensors, which detect mediated currents (i.e., the reduction current of the oxidized form of the mediators), the present HRP/Th-CF-based H₂O₂-biosensor directly detects the reduction current of the oxidative HRP-intermediates (i.e., compound I and II) based on a direct electron transfer (DET) between a HRP-redox active heme center and the CF electrode, because co-adsorbed Th together with HRP facilitate the DET. Various adsorption conditions (i.e., HRP concentration, Th concentration, ionic strength, pH, adsorption time) were optimized to obtain excellent sensor performances. When an air-saturated 0.1 M phosphate buffer (pH 7.0) was used as a carrier at 3.25 ml/min and the applied potential of – 0.05 V vs. Ag/AgCl, the HRP/Th-CF-based flow-H₂O₂-biosensor exhibited excellent analytical performances (i.e., sensitivity, 3.55 μA/μM; linear range, 0.1 to 10 μM; lower detection limit, 0.03 μM; K_m^app, 36.2 μM; I_max, 158 μM; sample throughput, ca. 60 samples/h).

収録刊行物

• Analytical Sciences

  Analytical Sciences 27 (4), 401-, 2011

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390001204260872960
• NII論文ID: 130004442010
• NII論文ID: 10028203517
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.27.401
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 11042263
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11042263
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.27.401.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.27.401/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/27/4/27_4_401/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可