Bifunctional Tri(ethylene glycol) Alkanethiol Monolayer Modified Gold Electrode for On-Chip Electrochemical Immunoassay of pg Level Leptin

• NISHIMURA Tetsuya

  National Institute of Advanced Industrial Science and Technology (AIST) Graduate School of Pure and Applied Science, University of Tsukuba

• SATO Yukari

  National Institute of Advanced Industrial Science and Technology (AIST)

• TANAKA Mutsuo

  National Institute of Advanced Industrial Science and Technology (AIST)

• KURITA Ryoji

  National Institute of Advanced Industrial Science and Technology (AIST)

• NAKAMOTO Kohei

  National Institute of Advanced Industrial Science and Technology (AIST) Graduate School of Pure and Applied Science, University of Tsukuba

• NIWA Osamu

  National Institute of Advanced Industrial Science and Technology (AIST) Graduate School of Pure and Applied Science, University of Tsukuba

抄録

An on-chip enzyme-linked immunosorbent assay combined with an electrochemical detection method (EC-ELISA) was employed to detect a leptin, one of the most important adipose derived hormones, using gold electrodes modified with a tri(ethylene glycol) terminated short alkanethiol (TEGCnSH, Cn = (CH₂)n, n = 2, 4, 6, and 8) monolayer. These TEGCnSH monolayers on gold electrodes can suppress non-specific protein adsorption without affecting the electrochemical activity required for detecting p-aminophenol (PAP), which is an alkaline phosphatase (ALP) product. We measured leptin with a highly sensitive detection range (100 pg mL⁻¹ to 10 ng mL⁻¹ level) and with the desired detection limit (13.6 pg mL⁻¹) by using electrochemical detection. For detecting leptin, the EC-ELISA method using TEGC4SH modified gold electrode with a poly(dimethylsiloxane) based microchannel was superior to the conventional ELISA method. With the EC-ELISA method, we were able to measure leptin with a satisfactory detection range and a pg level detection limit within 30 min, which is a much lower detection level than that obtained with conventional plate based ELISA.

収録刊行物

• Analytical Sciences

  Analytical Sciences 27 (5), 465-, 2011

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390282679237652992
• NII論文ID: 10028288506
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.27.465
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 11067849
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11067849
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.27.465.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.27.465/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/27/5/27_5_465/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012013532
• 本文言語コード: en
• データソース種別:
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