Carbon-felt-based bioelectrocatalytic flow-detectors: role of ultrasound irradiation during the adsorption of horseradish peroxidase and thionine for a highly sensitive amperometric determination of H2O2

• WANG Yue

  Department of Materials Science and Engineering, Graduate School of Engineering, Saitama Institute of Technology School of Chemical Engineering, University of Science and Technology Liaoning

• HASEBE Yasushi

  Department of Materials Science and Engineering, Graduate School of Engineering, Saitama Institute of Technology Department of Life Science and Green Chemistry, Saitama Institute of Technology

書誌事項

タイトル別名

• Carbon-felt-based Bioelectrocatalytic Flow-detectors: Role of Ultrasound Irradiation during the Adsorption of Horseradish Peroxidase and Thionine for a Highly Sensitive Amperometric Determination of H₂O₂

抄録

This study reports on the first example, to our knowledge, of the usefulness of an ultrasound (US)-irradiation during an enzyme adsorption step, for enhancing the performance of a redox-enzyme-based amperometric biosensor. Horseradish peroxidase (HRP) and thionine (Th) were coadsorbed from a mixed aqueous solution of HRP and Th onto a carbon-felt (CF) under US-irradiation for 5 min with an ultransonic bath operating at 40 kHz frequency and 55 W of electric power output. The resulting HRP and Th-coadsorbed CF (HRP/Th-CF) was successfully used as a working electrode unit of a bioelectrocatalytic flow-detector for hydrogen peroxide (H₂O₂), which detects the cathodic peak currents based on the direct (unmediated) reduction of oxidized HRP intermediates at 0 V vs. Ag/AgCl. Compared with ordinary adsorption without US-irradiation, US-irradiation during the HRP adsorption step was effective to obtain highly sensitive peak current responses to H₂O₂. The measurements of electrochemical impedance spectroscopy and cyclic voltammetry suggested that the adsorption of HRP and Th under the US-irradiation provides a suitable interfacial microenvironment for a favorable orientation and conformation of an enzyme with active site available for both substrates and the electrode, which results in larger bioelectrocatalytic activity. The peak currents for H₂O₂ increased up to 3 × 10⁻⁶ M (sensitivity, 4.72 μA/μM) with a lower detection limit of 2 × 10⁻⁸ M (S/N = 3; current noise level, 0.03 μA).

収録刊行物

• Analytical Sciences

  Analytical Sciences 27 (6), 605-605, 2011

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390001204260894592
• NII論文ID: 130004827154
• NII論文ID: 10028289177
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.27.605
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 11106293
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11106293
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.27.605.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.27.605/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/27/6/27_6_605/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可