Epigenetically Immature Oocytes Lead to Loss of Imprinting During Embryogenesis
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- OBATA Yayoi
- Department of BioScience, Tokyo University of Agriculture
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- HIURA Hitoshi
- Department of BioScience, Tokyo University of Agriculture Present: Department of Obstetrics, Tohoku University Graduate School of Medicine
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- FUKUDA Atsushi
- Department of BioScience, Tokyo University of Agriculture
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- KOMIYAMA Junichi
- Department of BioScience, Tokyo University of Agriculture
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- HATADA Izuho
- Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University
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- KONO Tomohiro
- Department of BioScience, Tokyo University of Agriculture
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Loss of imprinting (LOI) is occasionally observed in human imprinting disorders. However, the process behind the LOI is not fully understood. To gain a better understanding, we produced embryos and pups from mouse oocytes that lacked a complete methylation imprint using a method that involved transferring the nuclei of growing oocytes into the cytoplasm of enucleated fully grown oocytes following in vitro fertilization (IVF). We then analyzed the imprinting statuses. Our findings show that the incomplete methylation imprint derived from growing oocytes results in epigenetic mosaicism or a loss of methylation imprint (LOM) at maternal alleles in embryos. In some embryos, both hypo- and hypermethylated maternal Kcnq1ot1 alleles were detected, whereas either hypo- or hypermethylated maternal Kcnq1ot1 alleles were detected in others. Such tendencies were also observed at the Igf2r and Mest loci. Gene expression levels of imprinted genes were linked with their methylation statuses in some but not all embryos. Possible explanations of the inconsistency between the data from DNA methylation and gene expression include epigenetic mosaicism in embryos. Pups were successfully produced from growing oocytes at a quite low frequency. They exhibited an obese phenotype and LOI with respect to Igf2r, Snrpn and Mest. Our finding suggests the possibility that LOI/LOM at maternal alleles in human concepti could be derived from epigenetically immature/mutated oocytes.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 57 (3), 327-334, 2011
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206337923712
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- NII論文ID
- 10029050351
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- NII書誌ID
- AA10936678
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- COI
- 1:STN:280:DC%2BC3Mnmt1GksQ%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 11131832
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- PubMed
- 21289466
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可