Bid and Bax Are Involved in Granulosa Cell Apoptosis During Follicular Atresia in Porcine Ovaries

DOI Web Site Web Site 被引用文献3件 参考文献93件
  • SAI Takafumi
    Animal Resource Science Center, The University of Tokyo
  • GOTO Yasufumi
    Animal Resource Science Center, The University of Tokyo
  • YOSHIOKA Ryousuke
    Animal Resource Science Center, The University of Tokyo
  • MAEDA Akihisa
    Animal Resource Science Center, The University of Tokyo
  • MATSUDA Fuko
    Animal Resource Science Center, The University of Tokyo
  • SUGIMOTO Miki
    Graduate School of Agriculture, Kyoto University
  • WONGPANIT Kannika
    Animal Resource Science Center, The University of Tokyo
  • JIN Hua-Zi
    Animal Resource Science Center, The University of Tokyo
  • LI Jun-You
    Animal Resource Science Center, The University of Tokyo
  • MANABE Noboru
    Animal Resource Science Center, The University of Tokyo Research Center for Food Safety, The University of Tokyo

この論文をさがす

抄録

More than 99% of follicles undergo "atresia" during follicular development and growth. Follicular atresia is predominantly regulated by granulosa cell apoptosis. However, the intracellular signaling pathway of apoptosis in granulosa cells has not been revealed. In the present study, we examined changes in the expression of BH3-interacting domain death agonist (Bid) and Bcl-2-associated X protein (Bax), which are considered to promote the cell death ligand/receptor-mediated process in mitochondrion-dependent type II apoptosis, in porcine granulosa cells during atresia. Levels of mRNA and protein of Bid and Bax were determined by the reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting techniques, respectively. Levels of Bid and Bax mRNA and protein were markedly increased in granulosa cells of early atretic follicles compared with those of healthy follicles. In situ hybridization and immunohistochemical staining revealed that mRNA and protein of Bid and Bax were present in the granulosa cells, though only traces were found in healthy follicles; however, strong staining was noted in atretic follicles. These results indicate that Bid and Bax appear to be signal transduction factors in granulosa cells during follicular atresia and appear to play proapoptotic roles and confirm that the porcine granulosa cell is a mitochondrion-dependent type II apoptotic cell.<br>

収録刊行物

被引用文献 (3)*注記

もっと見る

参考文献 (93)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ