An active part of Artemisia sacrorum Ledeb. suppresses gluconeogenesis through AMPK mediated GSK3β and CREB phosphorylation in human HepG2 cells

• YUAN Hai-Dan

  Department of Pharmacognosy, College of Pharmacy, Yan Bian University Department of Pharmacological Science, College of Pharmacy, Kyunghee University

• PIAO Guang-Chun

  Department of Pharmacognosy, College of Pharmacy, Yan Bian University

書誌事項

タイトル別名

• An Active Part of Artemisia sacrorum Ledeb. Suppresses Gluconeogenesis through AMPK Mediated GSK3.BETA. and CREB Phosphorylation in Human HepG2 Cells
• An active part of Artemisia sacrorum Ledeb suppresses gluconeogenesis through AMPK mediated GSK3 v and CREB phosphorylation in human HepG2 cells
• An Active Part of<i>Artemisia sacrorum</i>Ledeb. Suppresses Gluconeogenesis through AMPK Mediated GSK3β and CREB Phosphorylation in Human HepG2 Cells

抄録

In this study, we investigated the effects of a petroleum ether fraction of Artemisia sacrorum Ledeb. (Compositae) (PEASL) on glucose production through AMP-activated protein kinase (AMPK) activation in human HepG2 cells. PEASL significantly inhibited glucose production in a concentration-dependent manner, and this effect was reversed in the presence of compound C, a selective AMPK inhibitor. PEASL markedly induced the phosphorylation of AMPK and downstream acetyl-CoA carboxylase (ACC) in a time- and concentration-dependent manner. In addition, it markedly increased the phosphorylations of glycogen synthase kinase 3β (GSK3β) in a concentration-dependent manner. In contrast, cAMP reponse element binding protein (CREB), a key transcription factor for gluconeogenic enzyme phosphorylation, decreased in a concentration-dependent manner. PEASL downregulated the gluconeogenesis gene expression of peroxisome proliferation activated receptor-γ coactivator-1α (PGC-1α), phosphoenolpyruvate carboxykinase (PEPCK), and glucose-6-phosphatase (G6Pase) in a concentration-dependent manner. In addition, the gene expression of orphan nuclear receptor small heterodimer partner (SHP) increased, also in a concentration-dependent manner. These effects were also abolished by pretreatment with compound C, an AMPK inhibitor. This indicates that PEASL inhibited glucose production via the AMPK-GSK-CREB pathway in HepG2 cells, and these effects appeared to be capable of revealing anti-diabetic mechanism of PEASL in HepG2 cells.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 75 (6), 1079-1084, 2011

  公益社団法人 日本農芸化学会

キーワード

• <I>Artemisia sacrorum</I> Ledeb.
• AMP-activated protein kinase
• glucose production

詳細情報

• CRID: 1390001206478198272
• NII論文ID: 10029327928
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.100881
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 11131918
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11131918
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.100881
• Web Site: https://search.jamas.or.jp/link/ui/2011337553
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可