Expression of recombinant human interferon-γ with antiviral activity in the Bi-cistronic baculovirus-insect/larval system

• CHEN Wen-Shuo

  Institute of Molecular Medicine, National Tsing Hua University

• VILLAFLORES Oliver B.

  Department of Chemistry, Chung Yuan Christian University

• JINN Tzyy-Rong

  Graduate Institute of Chinese Medical Science, China Medical University

• CHAN Ming-Tsair

  Institute of BioAgricultural Sciences, Academia Sinica

• CHANG Yen-Chung

  Institute of Molecular Medicine, National Tsing Hua University

• WU Tzong-Yuan

  Department of Bioscience Technology, Chung Yuan Christian University R&D Center for Membrane Technology, Chung Yuan Christian University

書誌事項

タイトル別名

• Expression of Recombinant Human Interferon-.GAMMA. with Antiviral Activity in the Bi-Cistronic Baculovirus-Insect/Larval System
• Expression of recombinant human interferon g with antiviral activity in the Bi cistronic baculovirus insect larval system

抄録

A bi-cistronic baculovirus-insect/larval system containing a polyhedron promoter, an internal ribosome entry site (IRES), and an egfp gene was developed as a cost-effective platform for the production of recombinant human interferon gamma (rhIFN-γ). There was no significant difference between the amounts of rhIFN-γ produced in the baculovirus-infected Spodoptera frugiferda 21 cells grown in serum-free medium and the serum-supplemented medium, while the Trichoplusia ni (T. ni) and Spodoptera exigua (S. exigua) larvae afforded rhIFN-γ amounting to 1.08±0.04 and 9.74±0.35 μg/mg protein respectively. The presence of non-glycosylated and glycosylated rhIFN-γ was confirmed by immunoblot and lectin blot. The immunological activity of purified rhIFN-γ, with 96% purity by Nickel (II)-nitrilotriacetic acid (Ni-NTA) affinity chromatography, was similar to that commercially available. Moreover, the rhIFN-γ protein from T. ni had more potent antiviral activity. These findings suggest that this IRES-based expression system is a simple and inexpensive alternative for large-scale protein production in anti-viral research.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 75 (7), 1342-1348, 2011

  公益社団法人 日本農芸化学会

キーワード

• baculovirus
• bi-cistronic
• <I>Spodoptera exigua</I> larvae
• human interferon gamma
• antiviral activity

詳細情報

• CRID: 1390001206476927360
• NII論文ID: 10029329338
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.110107
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 11175106
• PubMed: 21737931
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11175106
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110107
• Web Site: https://search.jamas.or.jp/link/ui/2011339595
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可