A simple method for purifying undenatured miraculin from transgenic tomato fruit

• Duhita Narendra

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• Hiwasa Kyoko

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• Yoshida Shigeki

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• Ezura Hiroshi

  Graduate School of Life and Environmental Sciences, University of Tsukuba

抄録

The sweet taste-modifying protein miraculin has been successfully expressed in the tomato, a high-yield commercial plant (Sun et al. 2007). Previously proposed methods of purifying untagged miraculin from transgenic tomato fruit are laborious and time consuming and do not allow the separation of undenatured and denatured miraculin. Therefore, it is necessary to develop an efficient method of purifying undenatured miraculin from transgenic tomato fruit. In this study, we have demonstrated that the combined use of nickel-immobilized affinity chromatography and ion-exchange chromatography readily provides highly purified undenatured miraculin, as demonstrated by the observation of both a single peak from reverse phase high performance liquid chromatography and a single band from SDS-PAGE.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 28 (3), 281-286, 2011

  日本植物バイオテクノロジー学会

キーワード

• Miraculin
• nickel column
• taste-modifying protein
• tomato
• transgenic plants

詳細情報

• CRID: 1390282679306300800
• NII論文ID: 10029399345
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.11.0207a
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 11133750
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11133750
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可