Neurobehavioral changes and alteration of gene expression in the brains of metallothionein-1/2 null mice exposed to low levels of mercury vapor during postnatal development

• Yoshida Minoru

  Faculty of Human Health Science, Hachinohe University

• Honda Masako

  Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University

• Watanabe Chiho

  Department of Human Ecology, School of International Health, Graduate School of Medicine, University of Tokyo

• Satoh Masahiko

  Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University

• Yasutake Akira

  Biochemistry Section, National Institute for Minamata Disease, Minamata

書誌事項

タイトル別名

• Neurobehavioral changes and alteration of gene expression in the brains of metallothionein-I/II null mice exposed to low levels of mercury vapor during postnatal development

抄録

This study examined the neurobehavioral changes and alteration in gene expression in the brains of metallothionein (MT)-I/II null mice exposed to low-levels of mercury vapor (Hg⁰) during postnatal development. MT-I/II null and wild-type mice were repeatedly exposed to Hg⁰ at 0.030 mg/m³ (range: 0.023-0.043 mg/m³), which was similar to the current threshold value (TLV), for 6 hr per day until the 20th day postpartum. The behavioral effects were evaluated with locomotor activity in the open field (OPF), learning ability in the passive avoidance response (PA) and spatial learning ability in the Morris water maze (MM) at 12 weeks of age. Hg⁰-exposed MT-I/II null mice showed a significant decrease in total locomotor activity in females, though learning ability and spatial learning ability were not affected. Immediately after Hg⁰ exposure, mercury concentrations in the brain did not exceed 0.5 µg/g in any animals. Hg⁰ exposure resulted in significant alterations in gene expression in the brains of both strains using DNA microarray analysis. The number of altered genes in MT-I/II null mice was higher than that in wild-type mice and calcium-calmodulin kinase II (Camk2a) involved in learning and memory in down-regulated genes was detected. These results provide useful information to elucidate the development of behavioral toxicity following low-level exposure to Hg⁰.

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ 36 (5), 539-547, 2011

  一般社団法人　日本毒性学会

キーワード

• Mercury vapor
• Low-level exposure
• Behavior, Microarray
• Gene expression

詳細情報

• CRID: 1390282679879507072
• NII論文ID: 10029481154
• NII書誌ID: AN00002808
• DOI: 10.2131/jts.36.539
• COI: 1:CAS:528:DC%2BC3MXhsVylsb7P
• ISSN: 18803989
• ISSN: 03881350
• NDL書誌ID: 11270373
• PubMed: 22008530
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11270373
• Web Site: http://www.jstage.jst.go.jp/article/jts/36/5/36_5_539/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012272208
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可