MicroRNAs expression in the Ethylene Glycol Monomethyl Ether-induced testicular lesion

• Fukushima Tamio

  Biochemical Toxicology, School of pharmaceutical sciences, Showa University Shionogi Co, Ltd. Drug Safety Evaluation

• Taki Kenji

  Biochemical Toxicology, School of pharmaceutical sciences, Showa University Pfizer Japan Inc., Drug Safety R&D, Pfizer Global R&D

• Ise Ryota

  Biochemical Toxicology, School of pharmaceutical sciences, Showa University Drug Safety Research Laboratories, Shin Nippon Biomedical Laboratories (SNBL)

• Horii Ikuo

  Biochemical Toxicology, School of pharmaceutical sciences, Showa University Pfizer Japan Inc., Drug Safety R&D, Pfizer Global R&D

• Yoshida Takemi

  Pfizer Japan Inc., Drug Safety R&D, Pfizer Global R&D

抄録

Ethylene glycol monomethyl ether (EGME) induces testicular lesion in rats and human.  To investigate miRNAs expression in EGME testicular lesion, miRNA array assay and real-time RT-PCR analysis were conducted by using testis in rats treated with 50 and 2,000 mg/kg EGME for 6 and 24 hr. The expression of corresponding target gene for miRNAs was also examined. At 50 mg/kg, there were no changes in the gene expression and histopathological examination. At 2,000 mg/kg, slight decrease of phacytene spermatocytes with cell shrinkage and nucleus pyknosis at 6 hr and remarkable decrease (or cell death) of phacytene spermatocytes with Sertoli cell vacuolation at 24 hr were observed. After 24 hr, miR-449a and miR-92a decreased obviously and, miR-320, miR-134 and miR-188 increased, while only miR-760-5p increased after 6 hr. Above these miRNAs are reported to have an important role for spermatogenesis. The gene expression of Bcl-2, target for miR-449a, increased and therefore it is considered anti-apoptotic reaction has started in this stage. The expression of high mobility group AT-hook 2 (target of miR-92a) which regulates histone structure, was increased. Furthermore, histone deacethylase 4, targets for miR-320, was also affected. Above prohibiting apoptosis or activating epigenetic genes might be protective reaction to spermatocytes death under the miRNAs regulation in EGME testicular lesion.

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ 36 (5), 601-611, 2011

  一般社団法人　日本毒性学会

キーワード

• EGME
• miRNA
• Testicular lesion

詳細情報

• CRID: 1390001204902672512
• NII論文ID: 10029481384
• NII書誌ID: AN00002808
• DOI: 10.2131/jts.36.601
• ISSN: 18803989
• ISSN: 03881350
• NDL書誌ID: 11270385
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11270385
• Web Site: http://www.jstage.jst.go.jp/article/jts/36/5/36_5_601/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012272213
• 本文言語コード: en
• データソース種別:
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  • NDL
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  • CiNii Articles
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