Four different ways of alternative transcripts generation mechanism in ADRA1A gene

DOI Web Site Web Site 参考文献69件
  • Huh Jae-Won
    National Primate Research Center (NPRC), KRIBB
  • Kim Young-Hyun
    National Primate Research Center (NPRC), KRIBB Department of Functional Genomics, University of Science & Technology
  • Lee Sang-Rae
    National Primate Research Center (NPRC), KRIBB
  • Kim Dae-Soo
    National Primate Research Center (NPRC), KRIBB
  • Park Sang-Je
    National Primate Research Center (NPRC), KRIBB Division of Biological Sciences, College of Natural Sciences, Pusan National University
  • Kim Hyoungwoo
    National Primate Research Center (NPRC), KRIBB
  • Kim Ji-Su
    National Primate Research Center (NPRC), KRIBB
  • Song Bong-Seok
    National Primate Research Center (NPRC), KRIBB
  • Kim Heui-Soo
    Division of Biological Sciences, College of Natural Sciences, Pusan National University
  • Chang Kyu-Tae
    National Primate Research Center (NPRC), KRIBB

この論文をさがす

抄録

The ADRA1A (Alpha-1-adrenergic receptor) gene is one of the members of G protein-coupled receptor superfamily. Alternative splicing of this gene was known to generate four transcript variants which code four isoforms with various C-terminal regions. In this study, we conducted expression analysis and evolutionary characterization of alternative transcripts of the ADRA1A gene. In total, 10 alternative transcripts were identified using experimental approaches and in silico analysis. Among them, 6 alternative transcripts (T1, T2, T3, T4, T4-1, and T8) were validated by RT-PCR approaches and sequencing procedures. From the alternative splicing analysis, it could be assumed that there were three different alternative transcripts generation mechanisms and unknown mechanism. First one is the integration event of three different TEs (AluSc, L1MC5, and MIR3) as seen on the last exons of T3, T4, T4-1, T5, T6, and T7 transcripts. The second mechanism is a differential promoter usage on T8. The third one is a substitution event of the 3’ splicing site during the primate evolution on T3. The last one is an unknown mechanism which was identified in the T4-1 transcript. Therefore, alternative transcripts of human ADRA1A gene occurred by four different ways, such as integration of TEs, differential promoter usage, substitution of splicing sites, and unknown mechanism.<br>

収録刊行物

参考文献 (69)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ