Genes for Difference in Eosinophilic Phenotype between MES and BN.MES-Cybames Rats Are on Chromosomes 9, 5, and 1

• TOMOZAWA Hiroshi

  Division of Laboratory Animal Research, Research Center for Human and Environmental Sciences, Shinshu University

• NISHIO Ayako

  Division of Laboratory Animal Research, Research Center for Human and Environmental Sciences, Shinshu University

• HIGUCHI Keiichi

  Department of Aging Biology, Institute on Aging and Adaptation, Shinshu University Graduate School of Medicine

• MATSUMOTO Kiyoshi

  Division of Laboratory Animal Research, Research Center for Human and Environmental Sciences, Shinshu University

• MORI Masayuki

  Department of Aging Biology, Institute on Aging and Adaptation, Shinshu University Graduate School of Medicine

抄録

The Matsumoto Eosinophilia Shinshu (MES) rat strain develops hereditary blood eosinophilia due to the mutant Cyba^mes gene. In contrast, BN.MES-Cyba^mes congenic rats, in which the mutant Cyba^mes gene introduced into the background of the BN strain, have a normal blood eosinophil level despite showing robust proliferation of eosinophils in the bone marrow. However, the congenic rats manifest focal necrosis with eosinophilic infiltration in the liver, a phenotype rarely observed in the original MES rat strain. To elucidate the genetic basis for the strain differences, (MES × BN.MES-Cyba^mes)F₂ rats were bred, and genetic analyses of phenotypes for eosinophilia were performed. Blood and bone marrow eosinophil levels in the F₂ rats showed broad distributions, suggesting that the traits were under the influence of multiple genes. Genetic association studies revealed that BN-derived marker loci on chromosomes 9 and 5 were responsible for the increase in eosinophil level in the bone marrow, decrease in blood eosinophil level, and the induction of focal necrosis with eosinophilic infiltration in the liver. The BN-derived allele of the marker gene on chromosome 1 was responsible for the decrease of both bone marrow and blood eosinophil levels. These data suggest the existence of genes characterizing/distinguishing the eosinophilic phenotypes of MES and BN.MES-Cyba^mes on these chromosomes, and form the basis for positional cloning studies of the genes. These studies will advance the understanding of the mechanisms involved in eosinophil mobilization from the bone marrow and recruitment to the organs.<br>

収録刊行物

• Experimental Animals

  Experimental Animals 60 (2), 151-160, 2011

  公益社団法人 日本実験動物学会

キーワード

• <i>Cyba</i>
• eosinophilia
• genetic polymorphism
• NADPH oxidase
• rat strain

詳細情報

• CRID: 1390001205045027712
• NII論文ID: 10029550320
• NII書誌ID: AA11032321
• DOI: 10.1538/expanim.60.151
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL書誌ID: 11036657
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11036657
• Web Site: http://www.jstage.jst.go.jp/article/expanim/60/2/60_2_151/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2011336801
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可