Complex Formation, Phosphorylation, and Localization of Protein Kinase A of<i>Schizosaccharomyces pombe</i>upon Glucose Starvation

• GUPTA Dipali Rani

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University

• PAUL Swapan Kumar

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University

• OOWATARI Yasuo

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University

• MATSUO Yasuhiro

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University

• KAWAMUKAI Makoto

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University

書誌事項

タイトル別名

• Complex Formation, Phosphorylation, and Localization of Protein Kinase A of Schizosaccharomyces pombe upon Glucose Starvation

抄録

Nine sam mutants that undergo sexual differentiation without requiring starvation in Schizosaccharomyces pombe were previously isolated. In this study, we identified a nonsense mutation on the pka1 locus in the sam6 mutant. pka1 encodes a catalytic subunit of protein kinase A (PKA). Replacement and overexpression of pka1 suppressed the KCl sensitivity and hyper-mating phenotype of sam6, confirming that sam6 is an allele of pka1. To characterize further the regulation of Pka1, we tested the physical interaction between Pka1 and Cgs1 (a regulatory subunit of PKA). Pka1 and Cgs1 physically interacted under glucose-limited conditions but not under glucose-rich conditions. In addition, the formation of a Pka1-Cgs1 complex was detected under glucose-limited conditions by Blue Native PAGE. Furthermore, the Pka1 protein was found to be phosphorylated under glucose-starved conditions, and at the same time its localization shifted from the nucleus towards the cytoplasm (mainly the vacuoles), suggesting a strong relationship among phosphorylation, complex formation, and the cytoplasmic distribution of Pka1.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 75 (8), 1456-1465, 2011

  公益社団法人 日本農芸化学会

キーワード

• fission yeast
• <I>Schizosaccharomyces pombe</I>
• protein kinase A
• sexual differentiation

詳細情報

• CRID: 1390001206479793664
• NII論文ID: 10029591165
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.110125
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 11222353
• PubMed: 21869531
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11222353
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110125
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可