Localization of a Novel RNA-binding Protein, SKIV2L2, to the Nucleus in the Round Spermatids of Mice

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  • OSMAN Boran A.H.
    Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki 305-8575, Japan
  • KAWASHIMA Akihiro
    Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki 305-8575, Japan
  • TAMBA Michiko
    Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki 305-8575, Japan
  • SATOH Emiko
    Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki 305-8575, Japan
  • KATO Yuhki
    Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki 305-8575, Japan
  • IKI Ayumi
    Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki 305-8575, Japan
  • KONISHI Kouhei
    Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki 305-8575, Japan
  • MATSUDA Manabu
    Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki 305-8575, Japan
  • OKAMURA Naomichi
    Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki 305-8575, Japan Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan

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In our previous study (Kawashima et al., Biol Reprod 2009; 80: 1293-1304), we suggested that the first cycle of spermatogenesis recovered from busulfan-induced temporary arrest was a good model system to analyze the proteins expressed at the specific stages of spermatogenesis in the mouse, and this has been confirmed in the present paper. Namely, six-week-old mice were injected with busulfan at 20 mg/kg body weight. The germ cells except for the undifferentiated spermatogonia disappeared by 32 days after injection. The surviving spermatogonia started to proliferate, and spermatogenesis was entirely recovered about 77 days after injection. By proteome analysis of the busulfan-treated testis during the process of recovery of spermatogenesis, we identified a protein that was expected to be expressed in the spermatogenic cells as Superkiller viralicidic activity-2-like-2 (SKIV2L2). Skiv2l2 mRNA was found in the kidney, epididymis and heart as well as the testis. In the testis, Skiv2l2 mRNA was shown to be highly expressed in the spermatocytes at stages I to VI. On the other hand, SKIV2L2 protein was found to be predominantly localized in the nuclei of round spermatids. In accordance with the fact that SKIV2L2 belongs to the Ski2 family within the superfamily 2 of RNA helicases, it has been shown that SKIV2L2 has both the RNA-binding and ATPase activities.<br>

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