An Enzymatic Colorimetric Quantification of Orthophosphate

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  • Li Bingxue
    National Food Research Institute, National Agriculture and Food Research Organization Shenyang Agricultural University
  • Nihira Takanori
    National Food Research Institute, National Agriculture and Food Research Organization
  • Nakai Hiroyuki
    National Food Research Institute, National Agriculture and Food Research Organization
  • Nishimoto Mamoru
    National Food Research Institute, National Agriculture and Food Research Organization
  • Kitaoka Motomitsu
    National Food Research Institute, National Agriculture and Food Research Organization

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Quantification of orthophosphate (Pi) in the presence of labile phosphate esters is required for biochemical assays. We developed a method for the enzymatic colorimetric quantification of Pi using pyruvate oxidase and peroxidase. The calibration curve was not affected by the presence of labile phosphate esters. Furthermore, this method allows continuous monitoring of the reaction of Pi-releasing enzymes.

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