Examination of transpositional activity of nDart1 at different stages of rice development
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- Hayashi-Tsugane Mika
- National Institute for Basic Biology Graduate School of Nutritional and Environmental Sciences, University of Shizuoka
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- Maekawa Masahiko
- Institute of Plant Science and Resources, Okayama University
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- Kobayashi Hirokazu
- Graduate School of Nutritional and Environmental Sciences, University of Shizuoka Global Center of Excellence Program, University of Shizuoka
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- Iida Shigeru
- National Institute for Basic Biology Graduate School of Nutritional and Environmental Sciences, University of Shizuoka Global Center of Excellence Program, University of Shizuoka Graduate School of Pharmaceutical Sciences, University of Shizuoka
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- Tsugane Kazuo
- National Institute for Basic Biology Graduate University for Advanced Studies (Sokendai)
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As a useful tool to elucidate gene functions, a rice transposon tagging line has been developed using an active endogenous DNA transposon, nDart1. It was highly desirable to evaluate the transposition timing and frequency of the nDart1 elements during rice development to facilitate the generation of an efficient mutant isolation system. Comparison of the detected new insertions at different stages of rice development by transposon display analysis demonstrated that the last heading tiller carry a higher number of nDart1 elements than the main culm. Moreover, it was revealed that the last heading tiller could produce progeny that carried more new insertions of nDart1 elements, mainly as a result of the accumulation of somatic insertions in the parental plant. This report demonstrates that late tillers increase the probability of producing independent mutant lines.<br>
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 86 (3), 215-219, 2011
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390282680449308672
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- NII論文ID
- 10029679006
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- NII書誌ID
- AA11077421
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- ISSN
- 18805779
- 13417568
- http://id.crossref.org/issn/13417568
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- NDL書誌ID
- 11238322
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可