UVC Mutagenicity Is Suppressed in Japanese Miso-Treated Human RSa Cells, Possibly<i>via</i>GRP78 Expression

• JIANG Xia

  Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University

• REN Qian

  Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University

• CHEN Shi-Ping

  Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University

• TONG Xiao-Bo

  Department of Physiology, Faculty of Basic Medical Sciences, Chengde Medical University

• DONG Mei

  Department of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine

• SUGAYA Shigeru

  Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University

• TANAKA Takeshi

  Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University

• KITA Kazuko

  Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University

• SUZUKI Nobuo

  Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University

書誌事項

タイトル別名

• UVC Mutagenicity Is Suppressed in Japanese Miso-Treated Human RSa Cells, Possibly via GRP78 Expression

抄録

Little is known about the ability of miso, to modulate mutability in human cells. We have observed increased levels of glucose-regulated protein 78 (GRP78) expression in association with suppression of mutation in human RSa cells irradiated with ultraviolet C (UVC). Here we examined to determine whether miso treatment results in increased GRP78 expression and suppression of UVC mutagenicity in RSa cells. Supernatants of water extracts of miso products and their components were tested. In the sample-treated cells, the amount of GRP78, as estimated by RT-PCR and immunoblotting analysis, increased, and the UVC-induced ouabain resistant mutation (Oua^R) and the K-ras codon 12-base substitution mutation frequency decreased. This decrease was not observed in cells with downregulation of GRP78 by GRP78 siRNA transfection. The results suggest that miso suppresses UVC mutagenicity by increasing GRP78 expression in human cells.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 75 (9), 1685-1691, 2011

  公益社団法人 日本農芸化学会

キーワード

• Japanese miso
• human RSa cells
• mutation
• ultraviolet C (UVC)
• glucose regulated protein 78 (GRP78)

詳細情報

• CRID: 1390001206479759360
• NII論文ID: 10029756890
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.110175
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 11252640
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11252640
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110175
• Web Site: https://search.jamas.or.jp/link/ui/2012048109
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可