Purification and Characterization of a Magnesium Ion Requiring<i>N</i>-Acetyl-<scp>D</scp>-glucosamine Specific Lectin from Seeds of<i>Quercus ilex</i>L.

• DEVI Sanjenbam Kunjeshwori

  Laboratory of Protein Biochemistry, Biochemistry Department, Manipur University

• SINGH Senjam Sunil

  Laboratory of Protein Biochemistry, Biochemistry Department, Manipur University

• SINGH Sorokhaibam Jibankumar

  Laboratory of Protein Biochemistry, Biochemistry Department, Manipur University

• RULLY Huidrom

  Laboratory of Protein Biochemistry, Biochemistry Department, Manipur University

• SINGH Laishram Rupachandra

  Laboratory of Protein Biochemistry, Biochemistry Department, Manipur University

書誌事項

タイトル別名

• Purification and Characterization of a Magnesium Ion Requiring N-Acetyl-D-glucosamine Specific Lectin from Seeds of Quercus ilex L.

抄録

A new magnesium ion requiring N-acetyl-D-glucosamine specific lectin QIL was purified to electrophoretic homogeneity from seeds of Quercus ilex L. through successive steps of (i) lectin extraction, (ii) ammonium sulphate (30–50%) fractionation, (iii) diethylaminoethyl (DEAE)-cellulose chromatography, (iv) carboxymethyl (CM)-cellulose chromatography, and (v) Sephadex G-75 chromatography. The lectin, having specific activity of 25,600 hemagglutination units (HAU)/mg of protein, was found to be a monomeric protein with a native molecular weight of 13.2 kDa. N-Acetyl-D-glucosamine was found to exhibit most potent inhibitory action on the lectin activity among all the sugars tested. The lectin was also found to exhibit specificity for human blood groups A, B, and AB. It was converted to the corresponding apo-lectin by ethylenediaminetetraacetic acid (EDTA) treatment followed by buffer dialysis. The apo-lectin exhibited a specific and characteristic requirement for magnesium ions for the expression of its activity.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 75 (9), 1752-1757, 2011

  公益社団法人 日本農芸化学会

キーワード

• lectin
• <I>N</I>-acetyl-<small>D</small>-glucosamine specific lectin
• magnesium ion requiring lectin
• <I>Quercus ilex</I> L.

詳細情報

• CRID: 1390282681457116416
• NII論文ID: 10029757280
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.110296
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 11252771
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11252771
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110296
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可