Comparison of Acyl-CoA Synthetic Activities and Enantioselectivity toward 2-Arylpropanoic Acids in Firefly Luciferases
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- KATO Dai-ichiro
- Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
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- YOKOYAMA Keisuke
- Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
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- HIRAISHI Yoshihiro
- Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
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- TAKEO Masahiro
- Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
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- NEGORO Seiji
- Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
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Measurement of thioesterification activities for dodecanoic acid (C12) and ketoprofen was done using five firefly luciferases, from Pyrocoelia miyako (PmL), Photinus pyralis (PpL), Luciola cruciata (LcL), Hotaria parvura (HpL), and Luciola mingrelica (LmL). Among these, PmL, PpL, and LcL showed the expected thioesterification activities toward both substrates. All the enzymes exhibited (R)-enantioselectivity toward ketoprofen, which had same tendency as firefly luciferase from Luciola lateralis (LUC-H). HpL and LmL, however, did not accept ketoprofen, although they had thioesterification activity toward C12. These results indicate that the substrate acceptance of luciferases for the thioesterification reaction varies dramatically relying on the origin of firefly. Hence we focused primarily on PmL and investigated the effect of pH on enzymatic activity. In addition, by determining the kinetic parameters at various pH values, we verified that the kcat parameter contributed to the preferential enantioselectivity of this enzyme.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 75 (9), 1758-1762, 2011
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206480405120
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- NII論文ID
- 10029757305
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3Mfmt1yhtw%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 11252777
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- PubMed
- 21897032
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可