A<i>Prunus mume</i>Extract Stimulated the Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells

• KONO Ryohei

  Department of Strategic Surveillance for Functional Food and Comprehensive Traditional Medicine, Wakayama Medical University Second Department of Internal Medicine, Wakayama Medical University

• OKUNO Yoshiharu

  Department of Materials Science, Wakayama National College of Technology

• INADA Ken-ichi

  Department of Pathology, Fujita Health University School of Medicine

• TOKUDA Akihiko

  Department of Strategic Surveillance for Functional Food and Comprehensive Traditional Medicine, Wakayama Medical University Second Department of Internal Medicine, Wakayama Medical University

• HASHIZUME Hiroshi

  Department of Orthopedic Surgery, Wakayama Medical University

• YOSHIDA Munehito

  Department of Orthopedic Surgery, Wakayama Medical University

• NAKAMURA Misa

  Department of Rehabilitation, Osaka Kawasaki Rehabilitation University

• UTSUNOMIYA Hirotoshi

  Department of Strategic Surveillance for Functional Food and Comprehensive Traditional Medicine, Wakayama Medical University Second Department of Internal Medicine, Wakayama Medical University

書誌事項

タイトル別名

• A Prunus mume Extract Stimulated the Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells

抄録

Osteoporosis is a serious disease caused by decreased bone mass. There is constant matrix remodeling in bones, by which bone formation is performed by osteoblastic cells, whereas bone resorption is accomplished by osteoclast cells. We investigated the effect of a Japanese apricot (Prunus mume SIBE. et ZUCC.) extract on the proliferation and osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. An alkaline phosphatase (ALP) activity assay, cell proliferation assay, alizarin red staining and expression analysis of osteoblastic genes were carried out to assess the proliferation and osteoblastic differentiation. The water-soluble fraction of Prunus mume (PWF) increased the ALP activity, cell proliferation and mineralization. The gene expression of osteopontin and bone morphogenetic protein-2, which are markers in the early period of osteoblastic differentiation, were significantly enhanced by the PWF treatment. PWF therefore stimulated the proliferation and osteoblastic differentiation of cells and may have potential to prevent osteoporosis.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 75 (10), 1907-1911, 2011

  公益社団法人 日本農芸化学会

キーワード

• osteoblastic differentiation
• <I>Prunus mume</I>
• MC3T3-E1
• osteoporosis
• bone

詳細情報

• CRID: 1390282681454525312
• NII論文ID: 10029872639
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.110264
• COI: 1:STN:280:DC%2BC3Mbjt1aksQ%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 11286346
• PubMed: 21979066
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11286346
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110264
• Web Site: https://search.jamas.or.jp/link/ui/2012054595
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可