A Six-well Plate Method: Less Laborious and Effective Method for Cultivation of Obligate Anaerobic Microorganisms

• Nakamura Kohei

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University

• Tamaki Hideyuki

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

• Kang Myung Suk

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) National Institute of Biological Resources

• Mochimaru Hanako

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

• Lee Sung-Taik

  Department of Biological Sciences, KAIST (Korea Advanced Institute of Science and Technology)

• Nakamura Kazunori

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

• Kamagata Yoichi

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Toyohira Research Faculty of Agriculture, Hokkaido University

抄録

We developed a simple, less laborious method to cultivate and isolate obligate anaerobic microorganisms using a six-well plate together with the AnaeroPack System, designated as the six-well plate method. The cultivation efficiency of this method, based on colony-forming units, colony formation time, and colony size, was evaluated with four authentic obligate anaerobes (two methanogenic archaea and two sulfate-reducing bacteria). The method was found to be comparable to or even better than the roll tube method, a technique that is commonly used at present for the cultivation of obligate anaerobes. Further experiments using 21 representative obligate anaerobes demonstrated that all examined anaerobes (11 methanogens, 5 sulfate- or thiosulfate-reducing bacteria, and 5 syntrophs) could form visible colonies on the six-well plate and that these colonies could be successfully subcultured in fresh liquid media. Using this method, an unidentified sulfate-reducing bacterium was successfully isolated from an environmental sample.<br>

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 26 (4), 301-306, 2011

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• methanogen
• sulfate-reducing bacterium (SRB)
• gellan gum
• uncultured anaerobes

詳細情報

• CRID: 1390282679322256128
• NII論文ID: 10029978006
• NII論文ID: 130000859554
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me11120
• COI: 1:STN:280:DC%2BC38%2Fjs1OktQ%3D%3D
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 023327779
• PubMed: 21685714
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023327779
• Web Site: http://www.jstage.jst.go.jp/article/jsme2/26/4/26_ME11120/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可