Retinoblastoma 94 Enhances Radiation Treatment of Esophageal Squamous Cell Carcinoma in Vitro and in Vivo

• ZHANG Heng

  Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine

• LI Jin

  Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine

• WANG Yue-ying

  Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine

• MENG Ai-min

  Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine

• LIU Qiang

  Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine

• WANG Lei

  Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine

• CHEN Feng-hua

  Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine

• WANG Xiao-chun

  Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine

• ZHAI Zhi-bin

  Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine

• FU Yue

  Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine

• WANG Qin

  Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine

抄録

We performed the study to investigate whether adenovirus-mediated retinoblastoma 94 (RB94) gene transfer could enhance radiation treatment of esophageal squamous cell carcinoma (ESCC) in vitro and in vivo. ESCC cells (Kyse150 cell line) were cultivated in vitro and tumors originated from the cell line were propagated as xenografts in nude mice. Treatment with Ad-RB94 and/or ionizing radiation (IR) was carried out both in vitro and in vivo with Ad-LacZ control vector and blank control. Cell viability, cell cycle distribution, cell apoptosis, tumor growth and transfected gene expression were evaluated and tumor degeneration was analyzed. The data of quantification real-time PCR assays and immunohistochemistry staining using RB antibody indicated that RB94 was efficiently transfected into Kyse150 cells. In vitro, data of cell growth assay indicated that treatment with Ad-RB94 improved radiation treatment of Kyse150 cells. Tumor xenograft studies, pathological analysis of H.E. staining and Ki67 staining suggested transfecting RB94 enhanced tumor regression induced by radiation treatment in vivo. In addition, data of Annexin V, TUNEL and cell cycle distribution assays proposed combination treatment effectively induced cell apoptosis and cell cycle arresting in G2/M phase. In conclusion, transferring RB94 gene by the adenoviral vector enhances radiation treatment of ESCC.

収録刊行物

• Journal of Radiation Research

  Journal of Radiation Research 53 (1), 117-124, 2012

  Journal of Radiation Research 編集委員会

キーワード

• Esophageal squamous cell carcinoma
• Radiotherapy
• Retinoblastoma 94
• Gene transfection
• Xenograft

詳細情報

• CRID: 1390282680194387840
• NII論文ID: 10030023777
• NII書誌ID: AA00705792
• DOI: 10.1269/jrr.11051
• ISSN: 13499157
• ISSN: 04493060
• NDL書誌ID: 023407880
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023407880
• Web Site: http://academic.oup.com/jrr/article-pdf/53/1/117/2706931/jrr-53-117.pdf
• Web Site: https://search.jamas.or.jp/link/ui/2013012102
• 本文言語コード: en
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