Identification of peptide motif that binds to the surface of zirconia

• HASHIMOTO Kazuhiko

  Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College Department of Clinical Pathophysiology, Tokyo Dental College Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research

• YOSHINARI Masao

  Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College

• MATSUZAKA Kenichi

  Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College Department of Clinical Pathophysiology, Tokyo Dental College

• SHIBA Kiyotaka

  Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research

• INOUE Takashi

  Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College Department of Clinical Pathophysiology, Tokyo Dental College

抄録

A zirconia-binding peptide motif was identified using a peptide phage display system. Yttria stabilized zirconia beads and discs were used as the target. Quartz crystal microbalance was used to monitor the binding of phages to zirconia. Starting from a library of phages displaying random sequences of 12-mer peptides, we repeated cycles of biopanning against zirconia beads. After four cycles of biopanning, we isolated a phage clone Φ#17. DNA sequencing of the corresponding portion of Φ#17 unexpectedly revealed that it displayed a 58-mer peptide (amino acid sequence: WMPSDVDINDPQGGGSRPNLHQPKPAAEAASKKKSENRKVPFYSHSWY-SSMSEDKRGW). We found that Φ#17 had a 300-fold, significantly higher binding affinity for zirconia discs than phages displaying no peptide. In quartz crystal microbalance assay, a rapid increase in energy dissipation was observed from Φ#17 but not from the control phages, indicating that Φ#17 binds to the surface of zirconia via its displayed peptide. We successfully identified a peptide motif that binds zirconia.

収録刊行物

• Dental Materials Journal

  Dental Materials Journal 30 (6), 935-940, 2011

  日本歯科理工学会

キーワード

• Phage display
• Peptide aptamer
• Zirconia

詳細情報

• CRID: 1390282679694794496
• NII論文ID: 10030026878
• NII書誌ID: AA10443149
• DOI: 10.4012/dmj.2011-161
• ISSN: 18811361
• ISSN: 02874547
• Web Site: https://www.jstage.jst.go.jp/article/dmj/30/6/30_2011-161/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012301061
• 本文言語コード: en
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