Comparison among amoA Primers Suited for Quantification and Diversity Analyses of Ammonia-Oxidizing Bacteria in Soil
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- Shimomura Yumi
- National Institute for Agro-Environmental Sciences
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- Morimoto Sho
- National Institute for Agro-Environmental Sciences
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- Takada Hoshino Yuko
- National Institute for Agro-Environmental Sciences
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- Uchida Yoshitaka
- National Institute for Agro-Environmental Sciences
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- Akiyama Hiroko
- National Institute for Agro-Environmental Sciences
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- Hayatsu Masahito
- National Institute for Agro-Environmental Sciences
書誌事項
- タイトル別名
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- Comparison among <i>amoA</i> Primers Suited for Quantification and Diversity Analyses of Ammonia-Oxidizing Bacteria in Soil
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抄録
Ammonia monooxygenase subunit A gene (amoA) is frequently used as a functional gene marker for diversity analysis of ammonia-oxidizing bacteria (AOB). To select a suitable amoA primer for real-time PCR and PCR-denaturing gradient gel electrophoresis (DGGE), three reverse primers (degenerate primer amoA-2R; non-degenerate primers amoA-2R-GG and amoA-2IR) were examined. No significant differences were observed among the three primers in terms of quantitative values of amoA from environmental samples using real-time PCR. We found that PCR-DGGE analysis with the amoA-2IR primer gave the best results in this studied soil. These results indicate that amoA-2IR is a suitable primer for community analysis of AOB in the environment.<br>
収録刊行物
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- Microbes and environments
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Microbes and environments 27 (1), 94-98, 2012
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001204345735936
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- NII論文ID
- 10030123028
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- NII書誌ID
- AA11551577
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- COI
- 1:STN:280:DC%2BC38zntlKjtg%3D%3D
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- ISSN
- 13474405
- 13426311
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- NDL書誌ID
- 023470184
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- PubMed
- 22075625
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可