Cardioprotective Effects of 3-Phosphoinositide-Dependent Protein Kinase-1 on Hypoxic Injury in Cultured Neonatal Rat Cardiomyocytes and Myocardium in a Rat Myocardial Infarct Model

• SHIM Tae-Jin

  Department of Internal Medicine, College of Medicine, Chungbuk National University College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University

• BAE Jang-Whan

  Department of Internal Medicine, College of Medicine, Chungbuk National University

• KIM Yeon-Jung

  Department of Internal Medicine, College of Medicine, Chungbuk National University

• KIM Dae Joong

  College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University

• HWANG Kyung-Kuk

  Department of Internal Medicine, College of Medicine, Chungbuk National University

• KIM Dong-Woon

  Department of Internal Medicine, College of Medicine, Chungbuk National University

• CHO Myeong-Chan

  Department of Internal Medicine, College of Medicine, Chungbuk National University

抄録

3-Phosphoinositide-dependent protein kinase-1 (PDK1) is involved in numerous cellular responses. In this study, we investigated the protective effects of PDK1 gene expression against hypoxic conditions in cultured rat CMCs (rCMCs) and in a rat myocardial infarction (MI) model using the lentiviral vector (LeV) system. LeV-PDK1 transfer effectively reduced the apoptotic cell death caused by hypoxic injury as compared to LeV-GFP transfer in rCMCs the expression of survival proteins increased in the LeV-PDK1 group, whereas apoptosis signaling decreased in the rCMCs and in infarcted hearts treated with LeV-PDK1. LeV-PDK1 transfer also reduced apoptosis and infarct size and attenuated myocardial wall thinning and ventricular remodeling in a rat MI model. These findings suggest that PDK1 has a protective role in the injured ischemic myocardium via overexpression of the cell survival pathway in CMCs. Hence PDK1 can be used as a treatment strategy for myocardial salvage inin hypoxic injury.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 76 (1), 101-107, 2012

  公益社団法人 日本農芸化学会

キーワード

• 3-phosphoinositide-dependent protein kinase-1
• gene expression
• cardiomyocytes
• myocardial infarction
• lentiviral vector

詳細情報

• CRID: 1390282681455217664
• NII論文ID: 10030398626
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.110562
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 023407631
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023407631
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110562
• Web Site: https://search.jamas.or.jp/link/ui/2012143766
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可