上皮膜蛋白輸送：胃粘液開口放出

• 中張 隆司

  大阪医科大学　中張プロジェクト（研究機構） 大阪医科大学　生理学教室

• 澤辺 幸紀

  大阪医科大学　中張プロジェクト（研究機構） 京都府立医科大学　大学院医学研究科　細胞生理学

• 島本 史夫

  大阪医科大学　中張プロジェクト（研究機構） 大阪薬科大学　薬物治療学II

書誌事項

タイトル別名

• Epithelial Transport of Macromolecules: Gastric Mucous Exocytosis
• ジョウヒマク タンパク ユソウ : イ ネンエキ カイコウ ホウシュツ

抄録

The mucosal surface of the gastric antrum was covered with mucous layers, which protect the mucosa from acidpeptic injuries. The mucous layers are maintained by the Ca²⁺- regulated exocytosis from antral mucous cells. The Ca²⁺- regulated exocytosis in antral mucous cells, which is stimulated by an increase in intracellular Ca²⁺ concentration ([Ca²⁺]_i), has a characteristic feature in the secretion pattern, that is, an initial transient phase (high frequency) followed by a sustained phase (low frequency). The final process of Ca²⁺- regulated exocytosis consists of three steps, docking, ATP-dependent priming and Ca²⁺- dependent fusion. Cyclic AMP (cAMP) increases the sensitivity to Ca²⁺ in the fusion step. Moreover, cAMP, cyclic GMP (cGMP) and a decrease in intracellular Cl^- concentration accelerate the priming step and increase the number of primed granules. This enhances the frequency of initial phase. In antral mucous cells, ACh released from vagal nerve endings induces an increase in [Ca²⁺]_i which stimulates arachidonic acid (AA) accumulation leading to prostaglandin E₂ (PGE₂) production. AA directly stimulates the peroxisome proliferation activation receptor α(PPAR α), which enhances the Ca²⁺. regulated exocytosis. PGE₂, which is immediately released from cells, stimulates EP4 receptors resulting in the enhancement of Ca²⁺- regulated exocytosis via cAMP accumulation in antral mucous cells. Thus, two autocrine mechanisms via PPAR α and EP4 activated by AA modulate Ca²⁺- regulated exocytosis in ACh-stimulated antral mucous cells and maintain massive mucous secretion in the gastric antrum.

収録刊行物

• 膜

  膜 36 (6), 286-292, 2011

  日本膜学会

キーワード

• gastric mucous secretion
• mucin
• Ca²⁺- regulated exocytosis
• prostaglandin E₂
• arachidonic acid
• PPAR α

詳細情報

• CRID: 1390001206423761920
• NII論文ID: 10030449887
• NII書誌ID: AN0023215X
• DOI: 10.5360/membrane.36.286
• COI: 1:CAS:528:DC%2BC38Xns1OmtQ%3D%3D
• ISSN: 18846440
• ISSN: 03851036
• NDL書誌ID: 023438601
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023438601
• Web Site: https://search.jamas.or.jp/link/ui/2012089019
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可