Assessment of technical protocols for novel embryonic stem cell tests with molecular markers (Hand1- and Cmya1-ESTs): a preliminary cross-laboratory performance analysis

• Suzuki Noriyuki

  Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd.

• Yamashita Norihisa

  Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd.

• Koseki Naoteru

  Safety Research Laboratories, Dainippon Sumitomo Pharma Co., Ltd.

• Yamada Toru

  Safety Research Laboratories, Dainippon Sumitomo Pharma Co., Ltd.

• Kimura Yutaka

  Department of Dermatology, Tohoku University Graduate School of Medicine

• Aiba Setsuya

  Department of Dermatology, Tohoku University Graduate School of Medicine

• Toyoizumi Tomoyasu

  Hatano Research Institute, Food and Drug Safety Center

• Watanabe Mika

  Hatano Research Institute, Food and Drug Safety Center

• Ohta Ryo

  Hatano Research Institute, Food and Drug Safety Center

• Tanaka Noriho

  Hatano Research Institute, Food and Drug Safety Center

• Saito Koichi

  Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd.

抄録

The Hand1- and Cmya1-ESTs are novel short-term tests for embryotoxic chemicals using genetically engineering mouse ES cells for luciferase reporter gene assays. These ESTs allow convenient determination of differentiation toxicity and cell viability in a short duration with high throughput 96-well microplates for prediction of embryotoxicity of chemicals. To assess the Hand1-EST technical protocol, we firstly compared reporter gene assay and cytotoxicity test data for a representative compound (hydroxyurea) from four different laboratories with tests carried out under the same experimental conditions. Extensive investigations of the Hand1- and Cmya1-ESTs were then performed to explore reproducibility by comparing a set of 6 well-known test chemicals, including hydroxyurea, across the laboratories. The results gave good correspondence in all four laboratories, indicating that transferability, intra-laboratory variability and inter-laboratory variability of the present technical protocols of the ESTs were sufficient to conduct further validation studies.

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ 37 (4), 845-851, 2012

  一般社団法人　日本毒性学会

キーワード

• In vitro
• Embryotoxicity
• Embryonic stem cell test
• Embryonic stem cells
• Differentiation
• Reporter gene assay

詳細情報

• CRID: 1390001204906512256
• NII論文ID: 10030502632
• NII書誌ID: AN00002808
• DOI: 10.2131/jts.37.845
• COI: 1:STN:280:DC%2BC38flsFartw%3D%3D
• ISSN: 18803989
• ISSN: 03881350
• NDL書誌ID: 023926310
• PubMed: 22863864
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023926310
• Web Site: https://www.jstage.jst.go.jp/article/jts/37/4/37_845/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2013306486
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可