DNA-binding and transcriptional activation properties of tobacco NIC2-locus ERF189 and related transcription factors

• Shoji Tsubasa

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• Hashimoto Takashi

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

書誌事項

タイトル別名

• DNA-binding and transcriptional activation properties of tobacco <i>NIC2</i>-locus ERF189 and related transcription factors

抄録

In tobacco (Nicotiana tabacum), several transcription factors belonging to the IXa group of APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) cluster at the regulatory NIC2 locus for the leaf nicotine level, and directly activate structural genes required for nicotine biosynthesis and transport. Solanaceous Atropa and Hyoscyamus plants synthesize medicinal tropane alkaloids by utilizing enzymes that partially overlap with the nicotine pathway. We tested whether the tobacco NIC2-locus ERF189 binds to potential ERF binding sites in the promoters of various structural genes involved in biosynthesis of nicotine and tropane alkaloids by an electrophoresis mobility shift assay. ERF189 bound four new GCC-box-like sequences in three nicotine structural genes, but did not recognize other candidate binding sites. This binding preference was used to optimize the consensus binding sequence of ERF189 as 5′-(A/C)GC(A/C)NNCC(A/T)-3′. Five group IXa ERFs (tobacco ERF189, tobacco ERF163, Catharanthus ORCA3, Arabidopsis AtERF13, and Arabidopsis AtERF1) were examined whether they bind to the ERF189-recognition site in the promoter of the tobacco putrescine N-methyltransferase gene in vitro, and transactivate the promoter in a transient expression assay using cultured tobacco cells. The more the protein sequences were similar to ERF189, the more effective these ERFs were in these functional assays. The transient expression assay also identified transactivation domains in an N-terminal acidic region of ERF189, and in a C-terminal Ser-rich region of AtERF13.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 29 (1), 35-42, 2012

  日本植物バイオテクノロジー学会

キーワード

• Alkaloids
• ERF transcription factors
• jasmonate
• nicotine
• tobacco

詳細情報

• CRID: 1390282679304403712
• NII論文ID: 10030561328
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.11.1216a
• COI: 1:CAS:528:DC%2BC38XmvFGmtr8%3D
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 023567385
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023567385
• Web Site: https://www.jstage.jst.go.jp/article/plantbiotechnology/29/1/29_11.1216a/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可