White clover mosaic virus-induced gene silencing in pea

• Ido, Yukari

• Nakahara, Kenji S

• Uyeda, Ichiro

抄録

Double-stranded RNAs formed in secondary structures and replicative intermediates of viral genomes are thought to strongly elicit RNA silencing. This phenomenon is known as virus-induced gene silencing (VIGS). VIGS is a powerful tool for modifying gene expression in host plants. We constructed a virus vector based on White clover mosaic virus (WClMV) and demonstrated VIGS of phytoene desaturase (PDS) in pea. Photobleaching of tissues, caused by VIGS of PDS, was observed in restricted areas of upper leaves and stems. We confirmed that the PDS mRNA and subgenomic RNAs of WClMV were reduced in the photobleached tissues.

収録刊行物

• Journal of General Plant Pathology

  Journal of General Plant Pathology 78 (2), 127-132, 2012

  Springer

キーワード

• Pea
• RNA silencing
• Potexvirus

詳細情報

• CRID: 1050564288964014080
• NII論文ID: 10030562130
• NII書誌ID: AA11449093
• DOI: 10.1007/s10327-012-0360-3
• ISSN: 1610739X
• ISSN: 13452630
• HANDLE: 2115/48569
• Web Site: http://link.springer.com/content/pdf/10.1007/s10327-012-0360-3.pdf
• Web Site: http://link.springer.com/article/10.1007/s10327-012-0360-3/fulltext.html
• Web Site: http://link.springer.com/content/pdf/10.1007/s10327-012-0360-3
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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