The Relationship between the Level of Progesterone Secreted from Cumulus Cells and Oocyte Developmental Competence in In Vitro Matured Human Cumulus Oocyte Complexes
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- Sato Kusaka Chikako
- Graduate School of Biosphere Science, Hiroshima University
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- Utsunomiya Takafumi
- St. Luke Clinic
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- Kumasako Yoko
- St. Luke Clinic
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- Otsu Eiko
- St. Luke Clinic
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- Mori Takahide
- Academia for Repro-regenerative Medicine
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- Shimada Masayuki
- Graduate School of Biosphere Science, Hiroshima University
書誌事項
- タイトル別名
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- The Relationship between the Level of Progesterone Secreted from Cumulus Cells and Oocyte Developmental Competence in <i>In Vitro</i> Matured Human Cumulus Oocyte Complexes
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Developmental competence of in vitro matured human oocytes is dependent on the morphology of the cumulus-oocyte complex (COC) just after collection from the follicle. We postulated that COCs categorized as having poor morphology (two or fewer less than two layers of cumulus cells) would not secrete a sufficient amount of matulation factors, resulting in low developmental competence of the matured oocytes. In the present study, the level of progesterone secreted from good morphology COCs with three or more layers of cumulus cells, 39.2 ± 12.8 ng/ml (n=31), was significantly higher than that secreted from poor morphology COCs (9.65 ± 1.34 ng/ml, n=22). The addition of 20 ng/ml progesterone to in vitro maturation culture of the poor morphology group significantly improved the fertilization ability of the oocytes. The rates of development to the morula and blastosyst stages were also increased by progesterone, however the differences were not significant. In conclusion, the secreted level of progesterone during in vitro maturation of human COCs was dependent on the number of cumulus cells attached to oocyte. When an oocyte is surrounded by two or fewer 2 layers of cumulus cells, the addition of progesterone to FSH- and hCG-containing medium appears to be a useful method for obtaining an oocyte with a high developmental competence.<br>
収録刊行物
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- 日本哺乳動物卵子学会誌
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日本哺乳動物卵子学会誌 29 (1), 41-47, 2012
日本卵子学会
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詳細情報 詳細情報について
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- CRID
- 1390001204338800768
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- NII論文ID
- 130004436829
- 10030590666
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- NII書誌ID
- AN10548943
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- ISSN
- 13475878
- 13417738
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- NDL書誌ID
- 023754035
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可