Reactivity of Direct Assays for Low-Density Lipoprotein (LDL) Cholesterol Toward Charge-Modified LDL in Hypercholesterolemia

DOI Web Site PubMed 被引用文献7件 参考文献38件
  • Zhang Bo
    Department of Biochemistry, Fukuoka University School of Medicine
  • Kawachi Emi
    Department of Cardiology, Fukuoka University School of Medicine
  • Matsunaga Akira
    Department of Laboratory Medicine, Fukuoka University School of Medicine
  • Imaizumi Satoshi
    Department of Cardiology, Fukuoka University School of Medicine
  • Noda Keita
    Department of Cardiology, Fukuoka University School of Medicine
  • Uehara Yoshinari
    Department of Cardiology, Fukuoka University School of Medicine
  • Miura Shin-ichiro
    Department of Cardiology, Fukuoka University School of Medicine
  • Yoshinaga Kazuhiko
    Department of Research Laboratory for Social Medicine, Fukuoka University School of Medicine
  • Kuroki Masahide
    Department of Biochemistry, Fukuoka University School of Medicine
  • Saku Keijiro
    Department of Cardiology, Fukuoka University School of Medicine

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Background: The aim of the present study was to compare 2 direct measurements for low-density lipoprotein cholesterol (LDL-C) with the Friedewald calculation (LDL-C [F]) in serum and their relationship with size-and charge-based LDL subfractions in serum ultracentrifugation fractions in patients with hypercholesterolemia (HC). Methods and Results: Serum samples from 283 HC patients who participated in a statin trial (the PATROL trial) were analyzed. Homogeneous assays for LDL-C were performed using reagents from Sekisui Medical (LDL-C [Se]) and Kyowa Medex (LDL-C [Ky]). Charge-based LDL subfractions were analyzed by capillary isotachophoresis (cITP). In whole serum in HC patients at baseline, LDL-C (Se) and LDL-C (Ky) negatively and positively deviated, respectively, from LDL-C (F). The negative deviation of LDL-C (Se) from LDL-C (F) increased with increasing LDL-C, while the positive deviation of LDL-C (Ky) from LDL-C (F) was positively correlated with charge-modified LDL (cmLDL) as analyzed by cITP. In serum d>1.006g/ml and >1.040g/ml fractions (LDL and small, dense LDL fractions, respectively), the deviation of LDL-C (Ky) from LDL-C (Se) was positively correlated with LDL-apoB (the number of LDL particles) and cmLDL. Conclusions: The 2 homogenous assays for LDL-C differed with regard to reactivity toward LDL particles and cmLDL in patients with HC. Direct measurement of LDL-C that reflects modified LDL, could be a better marker for the risk of coronary heart disease.  (Circ J 2012; 76: 2241–2248)<br>

収録刊行物

  • Circulation Journal

    Circulation Journal 76 (9), 2241-2248, 2012

    一般社団法人 日本循環器学会

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