Development of Simultaneous Enzymatic Assay Method for All Six Individual Vitamin B_6 Forms and Pyridoxine-β-Glucoside

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Author(s)

    • IDE Youhei
    • Faculty of Agriculture and Agricultural Science Program, Graduate School of Integrated Arts and Sciences, Kochi University
    • MUGO Andrew Njagi
    • Faculty of Agriculture and Agricultural Science Program, Graduate School of Integrated Arts and Sciences, Kochi University
    • YOSHIKANE Yu
    • Faculty of Agriculture and Agricultural Science Program, Graduate School of Integrated Arts and Sciences, Kochi University

Abstract

A method for determining all of the six natural vitamin B<sub>6</sub> compounds and pyridoxine-β-glucoside in urine from humans consuming their usual diet was developed. These compounds were specifically converted with 5 enzymes into a high fluorescent 4-pyridoxolactone which was supersensitively determined by an isocratic HPLC. All of the compounds in urine from humans consuming their usual diets were for the first time determined together. The preparation procedure for urine samples was easy without HCl-hydrolysis, and the enzyme reactions took only 2 or 3 h. It required only 5 μL of the urine sample for analysis of one of the compounds. Urine samples from five young Japanese males consuming their usual diet contained pyridoxal, pyridoxamine, and pyridoxine-β-glucoside but not pyridoxine or phosphoester forms. The contents of 4-pyridoxic acid and pyridoxal correlate well with a correlation coefficient of 0.98. On the other hand, the content of pyridoxamine did not correlate with that of 4-pyridoxic acid.

Journal

  • Journal of Nutritional Science and Vitaminology  

    Journal of Nutritional Science and Vitaminology 56(3), 157-163, 2010-06-01 

    Center for Academic Publications Japan

References:  18

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Cited by:  1

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Codes

  • NII Article ID (NAID)
    10030703710
  • NII NACSIS-CAT ID (NCID)
    AA00703822
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    03014800
  • NDL Article ID
    10715198
  • NDL Source Classification
    ZR2(科学技術--生物学--生化学)
  • NDL Call No.
    Z53-B484
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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