Comparative Proteomic Analysis of Liver Mitochondrial Proteins Derived from Cloned Adult Pigs Reconstructed with Meishan Pig Fibroblast Cells and European Pig Enucleated Oocytes
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- TAKEDA Kumiko
- National Agricultural and Food Research Organization (NARO), Institute of Livestock and Grassland Science (NILGS), Ibaraki 305-0901, Japan
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- TASAI Mariko
- National Agricultural and Food Research Organization (NARO), Institute of Livestock and Grassland Science (NILGS), Ibaraki 305-0901, Japan
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- IWAMOTO Masaki
- Prime Tech Ltd., Ibaraki, 300-0841, Japan
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- OE Mika
- National Agricultural and Food Research Organization (NARO), Institute of Livestock and Grassland Science (NILGS), Ibaraki 305-0901, Japan
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- CHIKUNI Koichi
- National Agricultural and Food Research Organization (NARO), Institute of Livestock and Grassland Science (NILGS), Ibaraki 305-0901, Japan
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- NAKAMURA Yoshiaki
- National Agricultural and Food Research Organization (NARO), Institute of Livestock and Grassland Science (NILGS), Ibaraki 305-0901, Japan Faculty of Agriculture, Shinshu University, Nagano 399-4598, Japan
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- TAGAMI Takahiro
- National Agricultural and Food Research Organization (NARO), Institute of Livestock and Grassland Science (NILGS), Ibaraki 305-0901, Japan
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- NIRASAWA Keijiro
- National Agricultural and Food Research Organization (NARO), Institute of Livestock and Grassland Science (NILGS), Ibaraki 305-0901, Japan
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- HANADA Hirofumi
- Laboratory of Animal Breeding, Tokyo University of Agriculture, Kanagawa 243-0034, Japan
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- PINKERT Carl A.
- Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849, USA
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- ONISHI Akira
- National Institute of Agrobiological Sciences (NIAS), Ibaraki 305-8602, Japan
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抄録
Somatic cell nuclear transfer (SCNT) has been exploited in efforts to clone and propagate valuable animal lineages. However, in many instances, recipient oocytes are obtained from sources independent of donor cell populations. As such, influences of potential nuclear-cytoplasmic incompatibility, post SCNT, are largely unknown. In the present study, alterations in mitochondrial protein levels were investigated in adult SCNT pigs produced by microinjection of Meishan pig fetus fibroblast cells into enucleated matured oocytes (maternal Landrace genetic background). Mitochondrial fractions were prepared from liver samples by mechanical homogenization and differential centrifugation. Liver mitochondria were then subjected to two-dimensional difference gel electrophoresis (2-D DIGE). Protein expression changes were confirmed with a volume ratio greater than 2 fold (P<0.05). 2-D DIGE analysis further revealed differential expression of three proteins between the Meishan (n=3) and Landrace (n=3) breeds. Differential expression patterns of 16 proteins were detected in SCNT pig liver tissue (n=3) when compared with Meishan control samples. However, none of the 16 proteins correlated with the three differentially expressed Meishan and Landrace liver mitochondrial proteins. In summary, alteration of mitochondrial protein expression levels was observed in adult SCNT pigs that did not reflect the breed difference of the recipient oocytes. Comparative proteomic analysis represents an important tool for further studies on SCNT animals.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 58 (2), 248-253, 2012
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206336097664
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- NII論文ID
- 10030754087
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- NII書誌ID
- AA10936678
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- COI
- 1:STN:280:DC%2BC38zptF2ltg%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 023602129
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- PubMed
- 22188878
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可