Induction of ski protein expression upon luteinization in rat granulosa cells without changes in its mRNA expression.
-
- KIM Hyun
- Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
-
- YAMANOUCHI Keitaro
- Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
-
- MATSUWAKI Takashi
- Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
-
- NISHIHARA Masugi
- Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
書誌事項
- タイトル別名
-
- Induction of Ski Protein Expression upon Luteinization in Rat Granulosa Cells without a Change in its mRNA Expression
この論文をさがす
抄録
The Ski protein is implicated in the proliferation/differentiation of a variety of cells. We previously reported that the Ski protein is present in granulosa cells of atretic follicles, but not in preovulatory follicles, suggesting that Ski has a role in apoptosis of granulosa cells. However, granulosa cells cannot only undergo apoptosis but can alternatively differentiate into luteal cells. It is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of the present study was to determine the localization of the Ski protein in the rat ovary during luteinization to examine if Ski might play a role in this process. In order to examine the Ski protein expression during the progression of luteinization, follicular growth was induced in immature female rats by administration of equine chorionic gonadotropin, and luteinization was induced by human chorionic gonadotropin treatment to mimic the luteinizing hormone (LH) surge. While no Ski-positive granulosa cells were present in the preovulatory follicle, Ski protein expression was induced in response to the LH surge and was maintained after formation of the corpus luteum (CL). Although the Ski protein is absent from the granulosa cells of the preovulatory follicle, its mRNA (c-ski) was expressed, and the level of c-ski mRNA was unchanged even after the LH surge. The combined results demonstrated that Ski protein expression is induced in granulosa cells upon luteinization, and suggested that its expression is regulated posttranscriptionally.
収録刊行物
-
- Journal of Reproduction and Development
-
Journal of Reproduction and Development 58 (2), 254-259, 2012
公益社団法人 日本繁殖生物学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001206336096384
-
- NII論文ID
- 130005457552
- 10030754124
-
- NII書誌ID
- AA10936678
-
- COI
- 1:STN:280:DC%2BC38vhtF2jtQ%3D%3D
-
- ISSN
- 13484400
- 09168818
-
- NDL書誌ID
- 023602137
-
- PubMed
- 22277932
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可