Development of transgenic plants in jatropha with drought tolerance

• Tsuchimoto Suguru

  Plant Bioengineering for Bioenergy Laboratory, Graduate School of Engineering, Osaka University

• Cartagena Joyce

  Bioengineering for the Interest of Environmental Sustainability (Sumitomo Electric Industries), Graduate School of Engineering, Osaka University

• Khemkladngoen Naruemon

  Bioengineering for the Interest of Environmental Sustainability (Sumitomo Electric Industries), Graduate School of Engineering, Osaka University

• Singkaravanit Suthitar

  Bioengineering for the Interest of Environmental Sustainability (Sumitomo Electric Industries), Graduate School of Engineering, Osaka University

• Kohinata Tsutomu

  Bioengineering for the Interest of Environmental Sustainability (Sumitomo Electric Industries), Graduate School of Engineering, Osaka University

• Wada Naoki

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Sakai Hiroe

  Plant Bioengineering for Bioenergy Laboratory, Graduate School of Engineering, Osaka University

• Morishita Yoshihiko

  Kazusa DNA Research Institute

• Suzuki Hideyuki

  Kazusa DNA Research Institute

• Shibata Daisuke

  Kazusa DNA Research Institute

• Fukui Kiichi

  Plant Bioengineering for Bioenergy Laboratory, Graduate School of Engineering, Osaka University Department of Biotechnology, Graduate School of Engineering, Osaka University

抄録

The seed oil of jatropha (Jatropha curcas L.) is a source of biodiesel fuel. Although jatropha can grow in semi-arid lands unsuitable for the food production, its oil productivity in such conditions is unsatisfactory at present. Therefore, it is desirable to improve the oil productivity of jatropha even in semi-arid lands by enhancing its drought tolerance. Genetic engineering is promising to dramatically improve plant traits. Although we previously reported a transformation method, which involves wounding of tissue explants in order to increase the chance of Agrobacterium infection, for jatropha, it remains a challenge to enhance the shoot regeneration and root induction processes. Here, we report the generation of three kinds of transgenic jatropha plants in an attempt to improve their drought tolerance. The first one overexpresses the PPAT gene, which encodes an enzyme that catalyzes the CoA biosynthetic pathway; the second overexpresses the NF-YB gene, which encodes a subunit of the NF-Y transcription factor; and the last overexpresses the GSMT and DMT genes, which encode enzymes that catalyze production of glycine betaine. We also report a modified protocol that improves the efficiency of shoot regeneration and root induction in transgenic jatropha plantlets.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 29 (2), 137-143, 2012

  日本植物バイオテクノロジー学会

キーワード

• Drought tolerance
• <i>Jatropha curcas</i> L.
• transgenic jatropha

詳細情報

• CRID: 1390001204327626752
• NII論文ID: 10030804213
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.12.0406d
• COI: 1:CAS:528:DC%2BC38Xht1Kksb%2FL
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 023783561
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023783561
• Web Site: https://www.jstage.jst.go.jp/article/plantbiotechnology/29/2/29_12.0406d/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可