Isolation and characterization of an asparagine-rich protein that regulates hypersensitive cell death-mediated resistance in Nicotiana plants

• Komori Daisuke

  Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University

• Nishihara Masahiro

  Iwate Biotechnology Research Center Iwate Biotechnology Research Center

• Takahashi Akiko

  Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University

• Gupta Meenu

  Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University

• Yoshioka Hirofumi

  Laboratory of Defense in Plant-Pathogen Interactions, Graduate School of Bioagricultural Sciences, Nagoya University Laboratory of Defense in Plant-Pathogen Interactions, Graduate School of Bioagricultural Sciences, Nagoya University

• Mizumoto Hiroyuki

  Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University

• Ohnishi Kouhei

  Research Institute of Molecular Genetics, Kochi University Research Institute of Molecular Genetics, Kochi University

• Hikichi Yusufumi

  Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University

• Kiba Akinori

  Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University

書誌事項

タイトル別名

• Isolation and characterization of an asparagine-rich protein that regulates hypersensitive cell death-mediated resistance in <i>Nicotiana</i> plants

抄録

We isolated and characterized Ralstonia solanacearum-responsive genes (RsRGs) related to the hypersensitive response (HR) by virus-induced gene silencing using Nicotiana benthamiana and the potato virus X vector system. We focused on RsRGM10, because induction of HR was delayed in RsRGM10-silenced plants challenged with incompatible R. solanacearum 8107, which induces the HR in, N. benthamiana. The amino acid sequence deduced from the full-length RsRGM10 cDNA showed approximately 90% identity with rolB overexpressed 1 (ROX1) from N. tabacum, and contained an asparagine-rich sequence. We designated this protein NbARP (N. benthamiana asparagine-rich protein). Expression of HR-related hin1 and onset of the oxidative burst were delayed in NbARP-silenced plants, and induction of myelin basic kinase activity was reduced. Growth of R. solanacearum 8107 was accelerated in NbARP-silenced plants. Induction of the HR by Pseudomonas cichorii and Pseudomonas syringae pv. syringae was also delayed in NbARP-silenced plants. Silencing of NbARP reduced induction of HR-cell death by Agrobacterium tumefaciens-mediated transient expression of HR elicitors and a constitutively active form of mitogen activated protein kinase kinase. The population of R. solanacearum 8107 was decreased in NbARP-overexpressing plants. These results suggest that NbARP is closely related to the HR.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 29 (3), 293-300, 2012

  日本植物バイオテクノロジー学会

キーワード

• Asparagine-rich protein
• Hypersensitive response
• <i>Nicotiana benthamiana</i>
• <i>Ralstonia solanacearum</i>

詳細情報

• CRID: 1390282679303617408
• NII論文ID: 10030804810
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.12.0213b
• COI: 1:CAS:528:DC%2BC38Xht1Chtb3P
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 023784545
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023784545
• 本文言語コード: en
• データソース種別:
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