Identification of a New IgE-Binding Epitope of Peanut Oleosin That Cross-Reacts with Buckwheat
-
- KOBAYASHI Shoko
- Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo
-
- KATSUYAMA Shinta
- Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo
-
- WAGATSUMA Tamae
- Department of Food and Life Science, Takasaki University of Health and Welfare
-
- OKADA Shinji
- Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
-
- TANABE Soichi
- Graduate School of Biosphere Science, Hiroshima University
この論文をさがす
抄録
Peanut and buckwheat induce a severe allergic reaction, anaphylaxis, which is considered to be mediated by immunoglobulin E (IgE). We identified in this study a new IgE-binding epitope of the peanut allergen that cross-reacted with buckwheat. The phosphate-buffered saline-soluble fraction of buckwheat inhibited the binding between IgE and the peanut allergen. A cross-reactive peptide was isolated from the α-chymotrypsin hydrolysate of peanut. Based on the amino acid sequence and mass spectrometric analysis data, the peptide was identified as Ser-Asp-Gln-Thr-Arg-Thr-Gly-Tyr (SDQTRTGY); this sequence is identical to amino acids 2–9 in the N-terminal hydrophilic domain of oleosin 3 which is located on the surface of the lipid storage body. Synthetic SDQTRTGY was found to bind with IgE in the sera of all eight peanut-allergic patients tested. Since many foods of plant origin contain oleosin, the possibility of an anaphylactic cross-reaction in allergic patients should always be considered.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 76 (6), 1182-1188, 2012
公益社団法人 日本農芸化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001206478067072
-
- NII論文ID
- 10030818299
-
- NII書誌ID
- AA10824164
-
- COI
- 1:STN:280:DC%2BC38jps1GgsQ%3D%3D
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 023768462
-
- PubMed
- 22790944
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可