Evaluation of the In Vivo Antioxidant Activity of Mucuna pruriens DC. var. utilis by Using Caenorhabditis elegans

• IBE Sachie

  Ohyamatofu Co., Ltd.

• FUJII Yoshiharu

  International Environmental Agriculture, Tokyo University of Agriculture and Technology

• OTOBE Kazunori

  National Agricultural Research Center, National Agriculture and Food Research Organization Graduate School of Life & Environmental Science, University of Tsukuba

書誌事項

タイトル別名

• Evaluation of the <i>In Vivo </i>Antioxidant Activity of <i>Mucuna pruriens</i> DC. var. <i>utilis</i> by Using <i>Caenorhabditis elegans</i>

抄録

Mucuna pruriens (MP) is a legume with seeds that contain substantial amounts of 3, 4-dihydroxy-L-phenylalanine (L-DOPA; 3% to 7% dry weight). We examined the in vivo antioxidant activity of distilled-water extracts of steamed or fermented MP seeds in Caenorhabditis elegans to evaluate the potential use of MP as a functional food. Paraquat and thermal stress generate reactive oxygen species in C. elegans. Worms treated with MP extracts were exposed to acute stress with paraquat or lethal heat shock. The survival rate 3 days after the treatment was used to indicate the antioxidant activity of the MP extracts. Distilled-water extracts of steamed or fermented MP had significantly higher (P < 0.01) antioxidant activity than in the control (nematode standard media) in vivo. L-DOPA showed high 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging activity but did not contribute to the antioxidant activity of MP in vivo.

収録刊行物

• Food Science and Technology Research

  Food Science and Technology Research 18 (2), 227-233, 2012

  公益社団法人 日本食品科学工学会

キーワード

• <i>Mucuna pruriens</i>
• antioxidant
• <small>L</small>-DOPA
• <i>Caenorhabditis elegans</i>

詳細情報

• CRID: 1390001204457318656
• NII論文ID: 10030917973
• NII書誌ID: AA11320122
• DOI: 10.3136/fstr.18.227
• COI: 1:CAS:528:DC%2BC38Xos1Orsrk%3D
• ISSN: 18813984
• ISSN: 13446606
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可