Survival of Bifidobacterium animalis subsp. lactis OPB-1 in the Gastrointestinal Tract after its Administration in a Milk-free Soybean Product and the Effect on Fecal Microbiota in Healthy Adults

• WATANABE Hisako

  Biwako Research Institute, Otsukafoods

• ISONO Yoshikazu

  Biwako Research Institute, Otsukafoods

書誌事項

タイトル別名

• Survival of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> OPB-1 in the Gastrointestinal Tract after its Administration in a Milk-free Soybean Product and the Effect on Fecal Microbiota in Healthy Adults

抄録

The survival of Bifidobacterium animalis subsp. lactis OPB-1 in the gastrointestinal tract after its administration in a milk-free yogurt-like soybean product and effects on fecal microbiota in humans were determined. Twelve healthy volunteers were administered 100 g of yogurt-like soybean product for 3 weeks. The plate count method combined with specific PCR and quantitative real-time PCR were used to estimate the numbers of total Bifidobacterium and B. animalis subsp. lactis in fecal samples. B. animalis subsp. lactis was detected during the administration period but not before. The intake number of B. animalis subsp. lactis OPB-1 was 2.4 × 10¹⁰ CFU/day by the plate count method and 4.2 × 10¹³ copies/day by quantitative real-time PCR. However, the number of excreted B. animalis subsp. lactis per day was estimated to be more than 10 times the intake number, by both methods. The percentage of Bifidobacterium increased from 16.9 ± 21.8% to 34.1 ± 20.4% by the plate count method and 18.9 ± 13.4% to 27.8 ± 15.8% by the T-RFLP method after 3 weeks administration.

収録刊行物

• Food Science and Technology Research

  Food Science and Technology Research 18 (2), 243-250, 2012

  公益社団法人 日本食品科学工学会

キーワード

• <i>Bifidobacterium animalis subsp. lactis</i>
• fecal microbiota
• soybean
• T-RFLP
• PCR

詳細情報

• CRID: 1390001204459804928
• NII論文ID: 10030918047
• NII書誌ID: AA11320122
• DOI: 10.3136/fstr.18.243
• COI: 1:CAS:528:DC%2BC38Xos1Ors78%3D
• ISSN: 18813984
• ISSN: 13446606
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可