Skeletal Muscle for Endomyocardial Biopsy: Comparable Stress Response in Doxorubicin Cardio-myopathy
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- Maita Rosa
- Sección de Biología Celular, Instituto de Medicina Tropical, Facultad de Medicina, Universidad Central de Venezuela
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- Strauss Mirian
- Sección de Biología Celular, Instituto de Medicina Tropical, Facultad de Medicina, Universidad Central de Venezuela
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- Anselmi Guillermo
- Sección de Biología Celular, Instituto de Medicina Tropical, Facultad de Medicina, Universidad Central de Venezuela
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In the present study, we compared the cell damage response in skeletal and cardiac muscle tissue when exposed to doxorubicin. This was carried out by means of a less invasive informative substitute to endomyocardiac biopsy based on Hsp70 immunodetection and a subcellular analysis of the nucleolus. Male Sprague Dawley rats (62 g body weight) were randomly distributed into 3 group, the control and doxorubicin I and doxorubicin II groups, in which 15 and 25 mg/kg body weight of doxorubicin (0.1 ml, i.v.) was administered, respectively. After 15, 30, 45 and 60 minutes, portions of the left and right ventricle wall and interventricle wall, together with skeletal muscle from the posterior and anterior member, were prepared for Hsp70 immunodetection by Western blot analysis and ultrastructural study using the thin cut technique. Differential cell response between the control and treated groups was observed in Hsp70 immunodetection and at the subcellular level. In the control group, the Hsp70 recognition levels and typical normal nucleolar morphology were similar, while the treated groups showed variable-dependent Hsp70 recognition and segregation of nucleolar components, forming ring-like figures of a variable-independent nature. Comparison of cardiac and skeletal muscle tissue cell response to doxorubicin toxic aggression revealed parallelism in terms of Hsp70 accumulation in certain regions of both tissues (15 mg/kg body weight of doxorubicin), which suggests that replacing endomyocardiac biopsy analysis with skeletal muscle analysis may be a safe option. <br>
収録刊行物
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- Journal of Toxicologic Pathology
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Journal of Toxicologic Pathology 22 (4), 273-279, 2009
日本毒性病理学会
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詳細情報 詳細情報について
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- CRID
- 1390001204416219904
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- NII論文ID
- 10030978392
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- NII書誌ID
- AN10232280
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- ISSN
- 1881915X
- 09149198
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- NDL書誌ID
- 10552488
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可