Sex Identification of Newly Hatched Chicks by Fluorescence in situ Hybridization using a W-specific DNA Probe in Feather Follicle Cells
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- H. Sohn Sea
- Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology, Republic of Korea Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology, Republic of Korea
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- J. Cho Eun
- Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology, Republic of Korea Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology, Republic of Korea
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- S. Kang Bo
- Poultry Science Division, National Institute of Animal Science, RDA, Republic of Korea Poultry Science Division, National Institute of Animal Science, RDA, Republic of Korea
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抄録
The identification of the sex of chickens or chicken cells has biological and industrial significance. In the laboratory, molecular techniques have utilized W chromosome-specific repetitive DNA sequence fragments, such as Xho I or EcoR I family, for gender identification in chickens. The present study examined a rapid and reliable fluorescence in situ hybridization (FISH) procedure for chick sex identification using an easily obtainable sample: uncultured feather pulp cells. A chicken W chromosome-specific 416-bp partial DNA fragment of Xho I family was digoxigenin-labeled by polymerase chain reaction and used as an FISH probe for chick sex identification. In 60 newly hatched chicks, 28 of the chicks that were subsequently identified as females by the visual identification of ovaries exhibited a positive W-specific probe signal in their feather follicle cells; the others were negative for the W-specific probe signal and were identified as males by the presence of testes. Therefore, the FISH results were in perfect agreement with the anatomical analysis. We suggest that this FISH technique using feather follicle cells and the W-specific DNA probe will be useful for a fast, convenient and accurate sex diagnosis of newly hatched chicks.
収録刊行物
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- The Journal of Poultry Science
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The Journal of Poultry Science 49 (4), 231-236, 2012
日本家禽学会
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詳細情報 詳細情報について
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- CRID
- 1390282680183924480
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- NII論文ID
- 10031120748
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- NII書誌ID
- AA11564513
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- COI
- 1:CAS:528:DC%2BC38XhvVelur3M
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- ISSN
- 13490486
- 13467395
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- NDL書誌ID
- 024025445
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可