Real-time PCR: Benefits for Detection of Mild and Asymptomatic Giardia Infections
-
- Prasertbun Rapeepun
- Department of Protozoology, Faculty of Tropical Medicine, Mahidol University
-
- Sukthana Yaowalark
- Department of Protozoology, Faculty of Tropical Medicine, Mahidol University
-
- Popruk Supaluk
- Department of Protozoology, Faculty of Tropical Medicine, Mahidol University
書誌事項
- タイトル別名
-
- Real-time PCR: Benefits for Detection of Mild and Asymptomatic <i>Giardia</i> Infections
この論文をさがす
抄録
The majority of Giardia infections are transmitted by the fecal-oral route and cause giardiasis. Children who live in crowded conditions or low socio-economic areas are the risk group for Giardia infection. Interestingly, most of them are asymptomatic or only mildly infected and can shed the Giardia cysts in the environment. Thus, the diagnosis of Giardia infection in asymptomatic or mild infection plays an important role in achieving control of Giardia duodenalis transmission. The objective of this study was to examine parasitic infections using microscopy and to develop a real-time PCR method for detection of Giardia infection in the stool samples of children living on the Thai-Myanmar border. Both species-specific primers and fluorescent labeled G. duodenalis probe were designed using small-subunit ribosomal RNA (ssrRNA). The results showed that 10 (7.69%) and 40 (30.77%) of 130 stool samples were positive for G. duodenalis by microscopy and real-time PCR respectively. Only 3 out of 9 liquid stools revealed G. duodenalis positive using microscopy, but all of them were G. duodenalis-positive using real-time PCR. The detection limit of real-time PCR for G. duodenalis was 0.1 pg/25 µl reaction. It can detect both mild and asymptomatic Giardia infections in children living on the Thai-Myanmar border.
収録刊行物
-
- Tropical Medicine and Health
-
Tropical Medicine and Health 40 (2), 31-35, 2012
日本熱帯医学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282680195568512
-
- NII論文ID
- 10031123155
-
- NII書誌ID
- AA11912846
-
- COI
- 1:STN:280:DC%2BC3s%2FotVKlug%3D%3D
-
- ISSN
- 13494147
- 13488945
-
- PubMed
- 23097617
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可