Expression of Avian Infectious Bronchitis Virus Multi-Epitope Based Peptide EpiC in Lactococcus lactis for Oral Immunization of Chickens

• CAO Hai-Peng

  Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environmental Protection of Southwestern China, Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environmental Protection of Southwestern China, Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University

• WANG Hong-Ning

  Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environmental Protection of Southwestern China, Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environmental Protection of Southwestern China, Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University

• ZHANG An-Yun

  Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environmental Protection of Southwestern China, Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environmental Protection of Southwestern China, Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University

• DING Meng-Die

  Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environmental Protection of Southwestern China, Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environmental Protection of Southwestern China, Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University

• LIU Si-Tong

  Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environmental Protection of Southwestern China, Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environmental Protection of Southwestern China, Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University

• CHENG Han

  Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environmental Protection of Southwestern China, Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environmental Protection of Southwestern China, Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University

• ZHOU Ying-Shun

  Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environmental Protection of Southwestern China, Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environmental Protection of Southwestern China, Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University

• LI Xin

  Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environmental Protection of Southwestern China, Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environmental Protection of Southwestern China, Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University

書誌事項

タイトル別名

• Expression of Avian Infectious Bronchitis Virus Multi-Epitope Based Peptide EpiC in <i>Lactococcus lactis</i> for Oral Immunization of Chickens

抄録

The avian infectious bronchitis virus (IBV) multi-epitope based peptide EpiC was found to be effective in inducing strong humoral and cellular responses against IBV. In this study, the gene EpiC was introduced into Lactococcus lactis NZ3900, and three recombinant strains expressing EpiC in intracellular and extracellular forms were constructed. SDS–PAGE and Western blot results indicated that EpiC was successfully expressed and had good immunoreactivity with chicken anti-IBV serum. Fusion of the signal pepitide gene SPusp45 and the nine-peptide LEISSTCDA encoding oligonucleotide to EpiC increased the secretion of EpiC, but reduced the total yields of EpiC. Oral immunization to specific-pathogen-free (SPF) chickens with recombinant strains induced significantly higher levels of humoral immune responses, and provided protection against lethal dose challenge by the IBV SAIBk strain. These results indicate that it is feasible to use L. lactis as an antigen delivery vehicle in developing oral vaccines against IBV infection.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 76 (10), 1871-1876, 2012

  公益社団法人 日本農芸化学会

キーワード

• avian infectious bronchitis virus
• multi-epitope
• intracellular
• extracellular
• <i>Lactococcus latis</i>

詳細情報

• CRID: 1390282681455067136
• NII論文ID: 130004054002
• NII論文ID: 10031126473
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.120326
• COI: 1:STN:280:DC%2BC3s%2FjsFGmsg%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 024035889
• PubMed: 23047098
• Web Site: http://id.ndl.go.jp/bib/024035889
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024035889
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.120326
• Web Site: https://search.jamas.or.jp/link/ui/2013215362
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可