Quick replication fork stop by overproduction of Escherichia coli DinB produces non-proliferative cells with an aberrant chromosome
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- Ikeda Mio
- Division of Systems Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Shinozaki Yutaka
- Division of Systems Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Uchida Kaori
- Division of Systems Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Ohshika Yasuha
- Division of Systems Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Furukohri Asako
- Division of Systems Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Maki Hisaji
- Division of Systems Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Akiyama Masahiro Tatsumi
- Division of Systems Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology
書誌事項
- タイトル別名
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- Quick replication fork stop by overproduction of <i>Escherichia coli</i> DinB produces non-proliferative cells with an aberrant chromosome
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抄録
Escherichia coli dinB encodes the translesion DNA polymerase DinB, which can inhibit progression of replication forks in a dose-dependent manner, independent of exogenous DNA damage. We reported previously that overproduction of DinB from a multicopy dinB plasmid immediately abolished ongoing replication fork progression, and the cells rapidly and drastically lost colony-forming ability, although the mechanisms underlying this lethality by severe replication fork stress remained unclear. Here, we show that the reduced colony-forming ability in the dinB-overexpressing cells is independent of the specific toxin genes that trigger programmed bacterial cell death when replication is blocked by depletion of the dNTP pool. After DinB abolished replication fork progression and colony-forming ability, most of the cells were still viable, as judged by fluorescent dye staining, but contained irregularly shaped nucleoids in which chromosomal DNA was preferentially lost in the replication terminus region relative to the replication origin region. Flow cytometric analysis of the cells revealed chromosomal damage and the eventual appearance of cell populations with less than single-chromosome DNA content, reminiscent of sub-G1 cells with lethal DNA content produced during eukaryotic apoptosis. This reduced DNA content was not observed after replication fork progression was quickly stopped in temperature-sensitive dnaB helicase mutant cells at a non-permissive temperature. Thus, the quick replication stop provoked by excess DinB uniquely generates temporarily viable but non-reproductive cells possessing a fatally depleted chromosomal content, which may represent one of the possible fates of an E. coli cell whose replication is overwhelmingly compromised.
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 87 (4), 221-231, 2012
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詳細情報 詳細情報について
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- CRID
- 1390001205472512640
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- NII論文ID
- 10031126615
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- NII書誌ID
- AA11077421
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- COI
- 1:STN:280:DC%2BC3s3htFOlsg%3D%3D
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 024078248
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- PubMed
- 23229309
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可