Expression profiles and unc-27 mutation rescue of the striated muscle type troponin I isoform-3 in Caenorhabditis elegans
-
- Takashima Yasuo
- Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
-
- Kitaoka Shun
- Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
-
- Bando Tetsuya
- Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
-
- Kagawa Hiroaki
- Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
書誌事項
- タイトル別名
-
- Expression profiles and <i>unc-27</i> mutation rescue of the striated muscle type troponin I isoform-3 in <i>Caenorhabditis elegans</i>
この論文をさがす
抄録
Transcription control of multiple genes in tissue- and stage-specific patterns is still of major interest. We show here that troponin I (TNI) is expressed under the control of upstream non-coding sequences and had functions as an isoform of intermediate type between pharynx and body-wall of the gene. In Caenorhabditis elegans, three striated muscle TNIs are expressed in body-wall muscles and a cardiac isoform is expressed in the pharynx. We have analyzed the gene expression mechanisms of tni-3 gene and motility function of its protein product. Promoter deletion analysis of the tni-3 gene identified muscle enhancers including the head enhancer. The CBF1/Su(H)/LAG-1-binding motif was included in the head enhancer. Yeast one-hybrid screening isolated the lag-1 clone in five candidates. Functional differences between the three striated muscle TNIs were investigated by the expression of promoter-fusion genes into tni-2/unc-27(e155) null mutant animals. The results suggest that the cis-elements in the promoters of the three genes are important for their tissue-specific expression and that from the function of TNI-3, the tni-3 gene would be an intermediate in the evolution of these genes by gene duplication. Mechanisms of tni-3 expression and its molecular function may contribute to our understanding of gene evolution and developmental programs.
収録刊行物
-
- Genes & Genetic Systems
-
Genes & Genetic Systems 87 (4), 243-251, 2012
日本遺伝学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282680449327104
-
- NII論文ID
- 10031126617
-
- NII書誌ID
- AA11077421
-
- COI
- 1:STN:280:DC%2BC3s3htFOlsA%3D%3D
-
- ISSN
- 18805779
- 13417568
-
- NDL書誌ID
- 024078291
-
- PubMed
- 23229311
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可