PARP and CSB modulate the processing of transcription-mediated DNA strand breaks
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- Sakai Akiko
- Innovative Anticancer Strategy for Therapeutics and Diagnosis Group, Innovation Center for Medical Redox Navigation, Kyushu University
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- Sakasai Ryo
- Innovative Anticancer Strategy for Therapeutics and Diagnosis Group, Innovation Center for Medical Redox Navigation, Kyushu University
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- Kakeji Yoshihiro
- Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University
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- Kitao Hiroyuki
- Innovative Anticancer Strategy for Therapeutics and Diagnosis Group, Innovation Center for Medical Redox Navigation, Kyushu University Department of Molecular Oncology, Graduate School of Medical Sciences, Kyushu University
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- Maehara Yoshihiko
- Innovative Anticancer Strategy for Therapeutics and Diagnosis Group, Innovation Center for Medical Redox Navigation, Kyushu University Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University
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抄録
Topoisomerase 1 (Top1)-DNA cleavage complexes induced by camptothecin (CPT) cause DNA strand breaks during DNA replication or transcription. Although the cellular responses to replication-mediated DNA double-strand breaks have been well studied, the responses to transcription-mediated DNA strand breaks have not. Here, we show that poly (ADP-ribose) polymerase (PARP) and cockayne syndrome group B protein (CSB) modulate the CPT-induced formation of discrete p53-binding protein 1 (53BP1) nuclear foci at sites of transcription-mediated DNA strand breaks. Inhibition of PARP activity enhanced the formation of these foci, while knockdown of essential components of the base excision repair (BER) pathway did not. These findings suggest that PARP suppresses transcription-mediated 53BP1 foci formation, but that this does not occur through the BER pathway. In addition, knockdown of CSB, one of the key factors of transcription-coupled repair, slowed the kinetics of 53BP1 foci formation. These data suggest that PARP and CSB modulate the formation of 53BP1 foci during the processing of transcription-mediated DNA strand breaks.
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 87 (4), 265-272, 2012
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390282680449319168
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- NII論文ID
- 10031126619
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- NII書誌ID
- AA11077421
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- COI
- 1:STN:280:DC%2BC3s3htFOltg%3D%3D
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 024078323
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- PubMed
- 23229313
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可