書誌事項
- タイトル別名
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- Observation of Cell Focal Adhesions Using Surface Plasmon Resonance Microscopy without Labeling
- ヒョウメン プラズモン ケンビキョウ ニ ヨル サイボウ セッチャク ブイ ノ ムヒョウシキ カンサツ
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Observation of cell adhesion site without chemical labeling contributes to the understanding of intracellular adhesion dynamics of biomolecules including integrin and other adhesion proteins. To satisfy the demands to investigate cell adhesion site without labeling, we employed a scanning localized surface plasmon microscopy (SLSPM) and observed the adhesion sites of the cell on a substrate with spatial resolution of 200 nm. By combining a non-scanning surface plasmon microscopy (SPM) and SLSPM, we observed a cell adhesion site and identifi ed the regions of interest. The main advantage of our system is that it has the highest spatial resolution among other SPM imaging systems, because size of an electric field distribution, which determines the spatial resolution, can be minimized by interference of surface plasmons excited with radially polarized light. In addition, we discuss the objectives to use the SLSPM and SPM systems reported by other groups for cell observation.
収録刊行物
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- レーザー研究
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レーザー研究 40 (12), 947-, 2012
一般社団法人 レーザー学会
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詳細情報 詳細情報について
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- CRID
- 1390285300184599296
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- NII論文ID
- 10031140361
- 130007891225
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- NII書誌ID
- AN00255326
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- ISSN
- 13496603
- 03870200
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- NDL書誌ID
- 024171033
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可