Functional Analysis of Lysosomes During Mouse Preimplantation Embryo Development

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  • TSUKAMOTO Satoshi
    Laboratory Animal and Genome Sciences Section, National Institute of Radiological Sciences, Chiba 263-8555, Japan
  • HARA Taichi
    Laboratory of Molecular Traffic, Institute for Molecular and Cellular Regulation, Gunma University, Gunma 371-8512, Japan
  • YAMAMOTO Atsushi
    Comprehensive Reproductive Medicine, Tokyo Medical and Dental University, Tokyo 113-8519, Japan Laboratory Animal and Genome Sciences Section, National Institute of Radiological Sciences, Chiba 263-8555, Japan
  • OHTA Yuki
    Science Service, Tokyo 103-0012, Japan
  • WADA Ayako
    Science Service, Tokyo 103-0012, Japan
  • ISHIDA Yuka
    Laboratory Animal and Genome Sciences Section, National Institute of Radiological Sciences, Chiba 263-8555, Japan
  • KITO Seiji
    Laboratory Animal and Genome Sciences Section, National Institute of Radiological Sciences, Chiba 263-8555, Japan
  • NISHIKAWA Tetsu
    Laboratory Animal and Genome Sciences Section, National Institute of Radiological Sciences, Chiba 263-8555, Japan
  • MINAMI Naojiro
    Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan
  • SATO Ken
    Laboratory of Molecular Traffic, Institute for Molecular and Cellular Regulation, Gunma University, Gunma 371-8512, Japan
  • KOKUBO Toshiaki
    Laboratory Animal and Genome Sciences Section, National Institute of Radiological Sciences, Chiba 263-8555, Japan

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Lysosomes are acidic and highly dynamic organelles that are essential for macromolecule degradation and many other cellular functions. However, little is known about lysosomal function during early embryogenesis. Here, we found that the number of lysosomes increased after fertilization. Lysosomes were abundant during mouse preimplantation development until the morula stage, but their numbers decreased slightly in blastocysts. Consistently, the protein expression level of mature cathepsins B and D was high from the one-cell to morula stages but low in the blastocyst stage. One-cell embryos injected with siRNAs targeted to both lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) were developmentally arrested at the two-cell stage. Pharmacological inhibition of lysosomes also caused developmental retardation, resulting in accumulation of lipofuscin. Our findings highlight the functional changes in lysosomes in mouse preimplantation embryos.

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