Design and Evaluation of PCR Primers for Denaturing Gradient Gel Electrophoresis Analysis of Plant Parasitic and Fungivorous Nematode Communities

• Kushida Atsuhiko

  Upland Farming Research Division, NARO Hokkaido Agricultural Research Center

抄録

A PCR-DGGE primer pair, Tyl2F-Tyl4R, specific to plant parasitic and fungivorous nematodes was designed based on the 18S rRNA gene. The results of community analysis using the primers showed that they are specific to the order Tylenchida. This primer pair detected species belonging to Tylenchida with high sensitivity and high resolution. The number of detected species of plant parasitic and fungivorous nematodes and their band intensity were much improved compared with PCR-DGGE analysis using the SSU18A-SSU9R primer, which is commonly used for nematode community analysis. It was confirmed that using a group-specific primer was effective for nematode community analysis with PCR-DGGE.<br>

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 28 (2), 269-274, 2013

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• detection sensitivity
• fungivorous nematode
• order <i>Tylenchida</i>
• PCR-DGGE primer
• plant parasitic nematode

詳細情報

• CRID: 1390282679322482944
• NII論文ID: 10031158632
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me12158
• COI: 1:STN:280:DC%2BC3srmtVKrtQ%3D%3D
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 024530284
• PubMed: 23595022
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024530284
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/28/2/28_ME12158/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可