Characterization of 5-Hydroxyconiferaldehyde O-Methyltransferase in Oryza sativa

DOI Web Site 被引用文献17件 参考文献61件
  • Koshiba Taichi
    Research Institute for Sustainable Humanosphere, Kyoto University
  • Hirose Norie
    Research Institute for Sustainable Humanosphere, Kyoto University
  • Mukai Mai
    Research Institute for Sustainable Humanosphere, Kyoto University
  • Yamamura Masaomi
    Research Institute for Sustainable Humanosphere, Kyoto University
  • Hattori Takefumi
    Research Institute for Sustainable Humanosphere, Kyoto University
  • Suzuki Shiro
    Research Institute for Sustainable Humanosphere, Kyoto University
  • Sakamoto Masahiro
    Graduate School of Agriculture, Kyoto University
  • Umezawa Toshiaki
    Research Institute for Sustainable Humanosphere, Kyoto University Institute of Sustainability Science, Kyoto University

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タイトル別名
  • Characterization of 5-Hydroxyconiferaldehyde <i>O</i>-Methyltransferase in <i>Oryza sativa</i>

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Little is known regarding syringyl lignin biosynthesis in rice (Oryza sativa L. cv. Nipponbare). In the present study, the role of rice caffeic acid O-methyltransferase (OsCOMT1, Q6ZD89), was examined. The recombinant OsCOMT1 catalyzed the 5-Omethylation of 5-hydroxyferulate (5-HFA) and 5-hydroxyconiferaldehyde (5-HCAld). 5-HCAld inhibited 5-HFA methylation by this O-methyltransferase (OMT), while 5-HFA mitigated self-inhibition in 5-HCAld methylation. A rice plant in which OsCOMT1 expression was downregulated exhibited weakened cell wall staining with Wiesner reagent in vascular bundle cells and sclerenchyma tissue, compared with wild-type plants. The lignin content of transgenic rice plants was decreased and the syringyl lignin content reduced largely compared with that of the wild type. Taken together, these data indicated that OsCOMT1 functioned as a 5-HCAld OMT (OsCAldOMT1) in the biosynthetic pathway to syringyl lignin.

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